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- PDB-2qxt: Crystal Structure Analysis of the Bacillus subtilis lipase crysta... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2qxt | ||||||
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Title | Crystal Structure Analysis of the Bacillus subtilis lipase crystallized at pH 4.5 | ||||||
![]() | Lipase | ||||||
![]() | HYDROLASE / alpha/beta hydrolase fold / Lipid degradation / Secreted | ||||||
Function / homology | ![]() lipase activity / triacylglycerol lipase / triglyceride lipase activity / lipid catabolic process / extracellular region Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Rajakumara, E. / Sankaranarayanan, R. | ||||||
![]() | ![]() Title: Structural basis for the remarkable stability of Bacillus subtilis lipase (Lip A) at low pH Authors: Rajakumara, E. / Acharya, P. / Ahmad, S. / Sankaranaryanan, R. / Rao, N.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 83.1 KB | Display | ![]() |
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PDB format | ![]() | 63.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 430.8 KB | Display | ![]() |
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Full document | ![]() | 436.3 KB | Display | |
Data in XML | ![]() | 18.2 KB | Display | |
Data in CIF | ![]() | 26.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2qxuC ![]() 1i6wS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 19182.643 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.05 Å3/Da / Density % sol: 39.98 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion / pH: 4.5 Details: 26% PEG 4000, 50mM Sodium Citrate pH 4.5, 50mM Ammonium Sulfate, VAPOR DIFFUSION, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Sep 10, 2003 / Details: mirrors |
Radiation | Monochromator: Osmic Mirror System / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2→25 Å / Num. all: 22197 / Num. obs: 21687 / % possible obs: 97.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.2 % / Biso Wilson estimate: 17.3 Å2 / Rsym value: 0.106 / Net I/σ(I): 11.1 |
Reflection shell | Resolution: 2→2.07 Å / Redundancy: 4 % / Mean I/σ(I) obs: 2.3 / Num. unique all: 2219 / Rsym value: 0.457 / % possible all: 95.4 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 1I6W Resolution: 2→24.94 Å / Rfactor Rfree error: 0.008 / Data cutoff high absF: 2543611.47 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 43.1144 Å2 / ksol: 0.347426 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 20.6 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2→24.94 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2→2.07 Å / Rfactor Rfree error: 0.036 / Total num. of bins used: 10
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Xplor file |
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