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Yorodumi- PDB-2q7n: Crystal structure of Leukemia inhibitory factor in complex with L... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2q7n | ||||||||||||
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Title | Crystal structure of Leukemia inhibitory factor in complex with LIF receptor (domains 1-5) | ||||||||||||
Components | (Leukemia inhibitory ...) x 2 | ||||||||||||
Keywords | CYTOKINE receptor/CYTOKINE / CYTOKINE CELL SURFACE RECEPTOR COMPLEX LIFR LIF / CYTOKINE receptor-CYTOKINE COMPLEX | ||||||||||||
Function / homology | Function and homology information leukemia inhibitory factor receptor binding / spongiotrophoblast differentiation / meiotic nuclear division / oncostatin-M receptor activity / positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis / IL-6-type cytokine receptor ligand interactions / leukemia inhibitory factor receptor activity / negative regulation of meiotic nuclear division / muscle organ morphogenesis / cell surface receptor signaling pathway via STAT ...leukemia inhibitory factor receptor binding / spongiotrophoblast differentiation / meiotic nuclear division / oncostatin-M receptor activity / positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis / IL-6-type cytokine receptor ligand interactions / leukemia inhibitory factor receptor activity / negative regulation of meiotic nuclear division / muscle organ morphogenesis / cell surface receptor signaling pathway via STAT / oncostatin-M-mediated signaling pathway / ciliary neurotrophic factor receptor binding / leukemia inhibitory factor signaling pathway / lung vasculature development / ciliary neurotrophic factor-mediated signaling pathway / regulation of metanephric nephron tubule epithelial cell differentiation / negative regulation of hormone secretion / trophoblast giant cell differentiation / positive regulation of peptidyl-serine phosphorylation of STAT protein / lung lobe morphogenesis / negative regulation of muscle cell apoptotic process / positive regulation of macrophage differentiation / positive regulation of astrocyte differentiation / IL-6-type cytokine receptor ligand interactions / cytokine receptor activity / positive regulation of cell adhesion mediated by integrin / lung alveolus development / cytokine binding / regulation of cell differentiation / growth factor binding / Interleukin-10 signaling / somatic stem cell population maintenance / macrophage differentiation / decidualization / neuron development / blood vessel remodeling / animal organ regeneration / positive regulation of tyrosine phosphorylation of STAT protein / embryo implantation / neuron projection morphogenesis / cytokine activity / response to cytokine / stem cell differentiation / growth factor activity / cell morphogenesis / negative regulation of ERK1 and ERK2 cascade / cytokine-mediated signaling pathway / positive regulation of fibroblast proliferation / positive regulation of peptidyl-tyrosine phosphorylation / positive regulation of peptidyl-serine phosphorylation / gene expression / fibroblast proliferation / Interleukin-4 and Interleukin-13 signaling / positive regulation of MAPK cascade / membrane => GO:0016020 / response to hypoxia / receptor complex / immune response / negative regulation of cell population proliferation / external side of plasma membrane / signaling receptor binding / positive regulation of cell population proliferation / positive regulation of gene expression / positive regulation of transcription by RNA polymerase II / extracellular space / extracellular region / cytosol Similarity search - Function | ||||||||||||
Biological species | Mus musculus (house mouse) Homo sapiens (human) | ||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 4 Å | ||||||||||||
Authors | Huyton, T. / Zhang, J.G. / Nicola, N.A. / Garrett, T.P.J. | ||||||||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2007 Title: An unusual cytokine:Ig-domain interaction revealed in the crystal structure of leukemia inhibitory factor (LIF) in complex with the LIF receptor. Authors: Huyton, T. / Zhang, J.G. / Luo, C.S. / Lou, M.Z. / Hilton, D.J. / Nicola, N.A. / Garrett, T.P. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2q7n.cif.gz | 281.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2q7n.ent.gz | 227.9 KB | Display | PDB format |
PDBx/mmJSON format | 2q7n.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/q7/2q7n ftp://data.pdbj.org/pub/pdb/validation_reports/q7/2q7n | HTTPS FTP |
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-Related structure data
Related structure data | 1pvhS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: 1 / Refine code: 5
NCS ensembles :
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-Components
-Leukemia inhibitory ... , 2 types, 4 molecules ACBD
#1: Protein | Mass: 55018.047 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Lifr / Plasmid: pCHO1/mLIFR / Production host: Cricetulus griseus (Chinese hamster) / Strain (production host): CHO-K1 / References: UniProt: P42703 #2: Protein | Mass: 19739.854 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: LIF, HILDA / Production host: Escherichia coli (E. coli) / References: UniProt: P15018 |
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-Sugars , 6 types, 18 molecules
#3: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta- ...2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source #4: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source #5: Polysaccharide | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1- ...alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | Source method: isolated from a genetically manipulated source #6: Polysaccharide | alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1- ...alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | Source method: isolated from a genetically manipulated source #7: Polysaccharide | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1- ...alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose | Source method: isolated from a genetically manipulated source #8: Sugar | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 7.866101 Å3/Da / Density % sol: 84.363281 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 1.5M sodium malonate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 0.968 Å |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Mar 30, 2005 / Details: mirrors |
Radiation | Monochromator: Si double crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.968 Å / Relative weight: 1 |
Reflection | Resolution: 4→20 Å / Num. all: 37104 / Num. obs: 37104 / % possible obs: 94.2 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 6.6 % / Rmerge(I) obs: 0.226 / Net I/σ(I): 6.3 |
Reflection shell | Resolution: 4→4.14 Å / Rmerge(I) obs: 0.857 / Mean I/σ(I) obs: 1 / % possible all: 72.5 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1PVH CHAIN B Resolution: 4→20 Å / Cor.coef. Fo:Fc: 0.9 / Cor.coef. Fo:Fc free: 0.868 / SU B: 91.108 / SU ML: 0.596 / TLS residual ADP flag: LIKELY RESIDUAL / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0.857 / ESU R Free: 0.663 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 102.084 Å2
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Refinement step | Cycle: LAST / Resolution: 4→20 Å
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Refine LS restraints |
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Refine LS restraints NCS | Dom-ID: 1 / Refine-ID: X-RAY DIFFRACTION
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LS refinement shell | Resolution: 4→4.105 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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