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Yorodumi- PDB-6cv7: Mouse Protocadherin-15 Extracellular Cadherin Domains 1 through 3 -
+Open data
-Basic information
Entry | Database: PDB / ID: 6cv7 | ||||||
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Title | Mouse Protocadherin-15 Extracellular Cadherin Domains 1 through 3 | ||||||
Components | Protocadherin-15 | ||||||
Keywords | CELL ADHESION / SIGNALING PROTEIN / Cadherin / Hair Cells / Hearing / Dimer | ||||||
Function / homology | Function and homology information detection of mechanical stimulus involved in equilibrioception / equilibrioception / sensory perception of light stimulus / inner ear receptor cell stereocilium organization / righting reflex / detection of mechanical stimulus involved in sensory perception of sound / inner ear auditory receptor cell differentiation / stereocilium / non-motile cilium assembly / photoreceptor cell maintenance ...detection of mechanical stimulus involved in equilibrioception / equilibrioception / sensory perception of light stimulus / inner ear receptor cell stereocilium organization / righting reflex / detection of mechanical stimulus involved in sensory perception of sound / inner ear auditory receptor cell differentiation / stereocilium / non-motile cilium assembly / photoreceptor cell maintenance / adult walking behavior / auditory receptor cell stereocilium organization / startle response / homophilic cell adhesion via plasma membrane adhesion molecules / inner ear development / photoreceptor outer segment / visual perception / locomotory behavior / actin filament organization / morphogenesis of an epithelium / sensory perception of sound / multicellular organism growth / response to calcium ion / cell adhesion / synapse / calcium ion binding / extracellular space / membrane / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.692 Å | ||||||
Authors | Dionne, G. / Shapiro, L. | ||||||
Citation | Journal: Neuron / Year: 2018 Title: Mechanotransduction by PCDH15 Relies on a Novel cis-Dimeric Architecture. Authors: Dionne, G. / Qiu, X. / Rapp, M. / Liang, X. / Zhao, B. / Peng, G. / Katsamba, P.S. / Ahlsen, G. / Rubinstein, R. / Potter, C.S. / Carragher, B. / Honig, B. / Muller, U. / Shapiro, L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6cv7.cif.gz | 179.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6cv7.ent.gz | 140.3 KB | Display | PDB format |
PDBx/mmJSON format | 6cv7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cv/6cv7 ftp://data.pdbj.org/pub/pdb/validation_reports/cv/6cv7 | HTTPS FTP |
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-Related structure data
Related structure data | 4apxS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 42812.824 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Pcdh15 / Cell line (production host): HEK293 / Production host: Homo sapiens (human) / References: UniProt: Q99PJ1 |
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-Sugars , 2 types, 8 molecules
#4: Sugar | ChemComp-MAN / #5: Sugar | |
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-Non-polymers , 3 types, 498 molecules
#2: Chemical | ChemComp-CA / #3: Chemical | ChemComp-EDO / #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.8 Å3/Da / Density % sol: 67.61 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: 35% Ethylene glycol |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.979 Å |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Apr 17, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 1.692→99.111 Å / Num. obs: 73884 / % possible obs: 99.26 % / Redundancy: 18.2 % / Biso Wilson estimate: 29.23 Å2 / CC1/2: 0.992 / Rmerge(I) obs: 0.1154 / Rrim(I) all: 0.1186 / Net I/σ(I): 20.19 |
Reflection shell | Resolution: 1.692→1.753 Å / Redundancy: 6.6 % / Rmerge(I) obs: 1.97 / Mean I/σ(I) obs: 0.69 / Num. unique obs: 6959 / CC1/2: 0.362 / % possible all: 92.5 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4APX Resolution: 1.692→99.111 Å / SU ML: 0.2 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 20.64
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.692→99.111 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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