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Yorodumi- PDB-2pn9: NMR structure of a kissing complex formed between the TAR RNA ele... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2pn9 | ||||||
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Title | NMR structure of a kissing complex formed between the TAR RNA element of HIV-1 and a LNA modified aptamer | ||||||
Components |
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Keywords | RNA / LNA / Locked Nucleic Acid / NMR / Aptamer / TAR / HIV-1 / kissing | ||||||
Function / homology | RNA / RNA (> 10) Function and homology information | ||||||
Method | SOLUTION NMR | ||||||
Authors | Lebars, I. / Richard, T. / Di Primo, C. / Toulme, J.-J. | ||||||
Citation | Journal: Nucleic Acids Res. / Year: 2007 Title: NMR structure of a kissing complex formed between the TAR RNA element of HIV-1 and a LNA-modified aptamer Authors: Lebars, I. / Richard, T. / Di Primo, C. / Toulme, J.-J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2pn9.cif.gz | 205.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2pn9.ent.gz | 169.9 KB | Display | PDB format |
PDBx/mmJSON format | 2pn9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2pn9_validation.pdf.gz | 338.1 KB | Display | wwPDB validaton report |
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Full document | 2pn9_full_validation.pdf.gz | 446 KB | Display | |
Data in XML | 2pn9_validation.xml.gz | 6.5 KB | Display | |
Data in CIF | 2pn9_validation.cif.gz | 10.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pn/2pn9 ftp://data.pdbj.org/pub/pdb/validation_reports/pn/2pn9 | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: RNA chain | Mass: 5168.134 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: RNA was prepared by in vitro transcription with T7 RNA polymerase |
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#2: RNA chain | Mass: 5136.132 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: Aptamer was purchased from Eurogentec |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: The structure was determined using standard homonulear and heteronuclear methods. |
-Sample preparation
Details |
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Sample conditions |
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-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 500 MHz |
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-Processing
NMR software |
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NMR representative | Selection criteria: closest to the average | ||||||||||||
NMR ensemble | Conformer selection criteria: The submitted structure are the 10 structures with zero violation on NOE distance, dihedral and with the lowest energy. Conformers calculated total number: 100 / Conformers submitted total number: 10 |