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- PDB-1mnb: BIV TAT PEPTIDE (RESIDUES 68-81), NMR, MINIMIZED AVERAGE STRUCTURE -

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Basic information

Entry
Database: PDB / ID: 1mnb
TitleBIV TAT PEPTIDE (RESIDUES 68-81), NMR, MINIMIZED AVERAGE STRUCTURE
Components
  • BIV TAR RNA
  • BIV TAT PEPTIDE
KeywordsViral protein/RNA / COMPLEX (REGULATORY PROTEIN-RNA) / TRANSCRIPTION REGULATION / Viral protein-RNA COMPLEX
Function / homology
Function and homology information


positive regulation of viral transcription / host cell nucleolus / RNA-binding transcription regulator activity / RNA binding
Similarity search - Function
Tat domain superfamily / Immunodeficiency virus transactivating regulatory protein (Tat) / Transactivating regulatory protein (Tat)
Similarity search - Domain/homology
RNA / RNA (> 10) / Protein Tat
Similarity search - Component
MethodSOLUTION NMR
AuthorsPuglisi, J.D. / Chen, L. / Blanchard, S. / Frankel, A.D.
CitationJournal: Science / Year: 1995
Title: Solution structure of a bovine immunodeficiency virus Tat-TAR peptide-RNA complex.
Authors: Puglisi, J.D. / Chen, L. / Blanchard, S. / Frankel, A.D.
History
DepositionJul 25, 1996Processing site: BNL
Revision 1.0Jan 27, 1997Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 23, 2022Group: Database references / Derived calculations / Other
Category: database_2 / pdbx_database_status ...database_2 / pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site
Revision 1.4May 22, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
B: BIV TAR RNA
A: BIV TAT PEPTIDE


Theoretical massNumber of molelcules
Total (without water)10,6532
Polymers10,6532
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)1 / -
Representative

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Components

#1: RNA chain BIV TAR RNA


Mass: 8923.310 Da / Num. of mol.: 1 / Fragment: RESIDUES 4 - 32 / Source method: obtained synthetically
#2: Protein/peptide BIV TAT PEPTIDE


Mass: 1730.083 Da / Num. of mol.: 1 / Fragment: RESIDUES 68 - 81
Source method: isolated from a genetically manipulated source
References: UniProt: P19564

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR detailsText: 50 MM NACL 10MM NA PHOSPHATE

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Sample preparation

Sample conditionspH: 6.4 / Temperature: 298 K
Crystal grow
*PLUS
Method: other / Details: NMR

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Processing

NMR softwareName: NMRARCHITECT, DISCOVER / Version: DISCOVER / Developer: BIOSYM / Classification: refinement
NMR ensembleConformers submitted total number: 1

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