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Open data
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Basic information
Entry | Database: PDB / ID: 2kjw | ||||||
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Title | Solution structure and backbone dynamics of the permutant P54-55 | ||||||
![]() | 30S ribosomal protein S6 | ||||||
![]() | RIBOSOMAL PROTEIN / S6 permutant / solution structure / backbone dynamics / folding / Ribonucleoprotein / RNA-binding / rRNA-binding | ||||||
Function / homology | ![]() small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / structural constituent of ribosome / translation Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model 1 | ||||||
![]() | Ohman, A. / Oliveberg, M. / Oman, T. | ||||||
![]() | ![]() Title: Solution structures and backbone dynamics of the ribosomal protein S6 and its permutant P(54-55) Authors: Ohman, A. / Oman, T. / Oliveberg, M. #1: Journal: Proc.Natl.Acad.Sci.USA / Year: 2009 Title: The HD-exchange motions of ribosomal protein S6 are insensitive to reversal of the protein-folding pathway Authors: Haglund, E. / Lind, J. / Oman, T. / Ohman, A. / Maler, L. / Oliveberg, M. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 620.2 KB | Display | ![]() |
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PDB format | ![]() | 519.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 345.6 KB | Display | ![]() |
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Full document | ![]() | 562.7 KB | Display | |
Data in XML | ![]() | 66.5 KB | Display | |
Data in CIF | ![]() | 88 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2kjvC C: citing same article ( |
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Similar structure data | |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 11258.886 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: Permutant of the ribosomal protein S6 from Thermus thermophilus Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR Details: Solution structure and backbone dynamics of the permutant S6p3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
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Sample |
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Sample conditions | pH: 6.3 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 600 MHz |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: sa.inp and refine.inp used with slight modifications. | ||||||||||||||||||||||||||||||||||||||||||
NMR constraints | NOE constraints total: 770 / NOE intraresidue total count: 213 / NOE long range total count: 116 / NOE medium range total count: 138 / NOE sequential total count: 303 / Protein phi angle constraints total count: 88 / Protein psi angle constraints total count: 65 | ||||||||||||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 200 / Conformers submitted total number: 20 |