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Yorodumi- PDB-2pgh: STRUCTURE DETERMINATION OF AQUOMET PORCINE HEMOGLOBIN AT 2.8 ANGS... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2pgh | |||||||||
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Title | STRUCTURE DETERMINATION OF AQUOMET PORCINE HEMOGLOBIN AT 2.8 ANGSTROM RESOLUTION | |||||||||
Components |
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Keywords | OXYGEN TRANSPORT | |||||||||
Function / homology | Function and homology information hemoglobin alpha binding / haptoglobin binding / haptoglobin-hemoglobin complex / organic acid binding / hemoglobin complex / hydrogen peroxide catabolic process / oxygen carrier activity / oxygen binding / peroxidase activity / blood microparticle ...hemoglobin alpha binding / haptoglobin binding / haptoglobin-hemoglobin complex / organic acid binding / hemoglobin complex / hydrogen peroxide catabolic process / oxygen carrier activity / oxygen binding / peroxidase activity / blood microparticle / iron ion binding / heme binding / metal ion binding Similarity search - Function | |||||||||
Biological species | Sus scrofa (pig) | |||||||||
Method | X-RAY DIFFRACTION / Resolution: 2.8 Å | |||||||||
Authors | Katz, D.S. / White, S.P. / Huang, W. / Kumar, R. / Christianson, D.W. | |||||||||
Citation | Journal: J.Mol.Biol. / Year: 1994 Title: Structure determination of aquomet porcine hemoglobin at 2.8 A resolution. Authors: Katz, D.S. / White, S.P. / Huang, W. / Kumar, R. / Christianson, D.W. #1: Journal: Cancer Detect.Prev. / Year: 1993 Title: Production of Hemoglobin in Transgenic Swine: An Approach to a Blood Substitute Authors: O'Donnell, J.K. / Martin, M.J. / Logan, J.S. / Kumar, R. #2: Journal: Biomater.,Artif.Cells, Immobilization Biotechnol. / Year: 1992 Title: The Purification and Comparative Analysis of Hemoglobin from Animal Bloods Authors: Lee, C.J. / Kan, P. / Chen, W.K. #3: Journal: Bio/Technology / Year: 1992 Title: Production of Functional Human Hemoglobin in Transgenic Swine Authors: Swanson, M.E. / Martin, M.J. / O'Donnell, J.K. / Hoover, K. / Sago, W. / Huntress, V. / Parsons, C.T. / Pinkert, C.A. / Pilder, S. / Logan, J.S. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2pgh.cif.gz | 126.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2pgh.ent.gz | 99.7 KB | Display | PDB format |
PDBx/mmJSON format | 2pgh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2pgh_validation.pdf.gz | 686.9 KB | Display | wwPDB validaton report |
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Full document | 2pgh_full_validation.pdf.gz | 723.3 KB | Display | |
Data in XML | 2pgh_validation.xml.gz | 18.9 KB | Display | |
Data in CIF | 2pgh_validation.cif.gz | 26.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pg/2pgh ftp://data.pdbj.org/pub/pdb/validation_reports/pg/2pgh | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 15064.144 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sus scrofa (pig) / Tissue: BLOOD / Organ: BLOOD / References: UniProt: P01965 #2: Protein | Mass: 16059.345 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sus scrofa (pig) / Tissue: BLOOD / Organ: BLOOD / References: UniProt: P02067 #3: Chemical | ChemComp-HEM / #4: Water | ChemComp-HOH / | Compound details | THE DEPOSITORS CONCLUDE THAT THEIR CRYSTALS ARE OF AQUOMET FORM BECAUSE: A) PRECAUTIONS SIMILAR TO ...THE DEPOSITORS | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.36 Å3/Da / Density % sol: 47.79 % | ||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 7 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | *PLUS Highest resolution: 2.8 Å / Num. obs: 13290 / Num. measured all: 44042 / Rmerge(I) obs: 0.087 |
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-Processing
Software |
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Refinement | Resolution: 2.8→6.5 Å / σ(F): 2 /
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Refinement step | Cycle: LAST / Resolution: 2.8→6.5 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.154 / Rfactor Rfree: 0.295 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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