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基本情報
登録情報 | データベース: PDB / ID: 2ohx | ||||||
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タイトル | REFINED CRYSTAL STRUCTURE OF LIVER ALCOHOL DEHYDROGENASE-NADH COMPLEX AT 1.8 ANGSTROMS RESOLUTION | ||||||
![]() | ALCOHOL DEHYDROGENASE | ||||||
![]() | OXIDOREDUCTASE(NAD(A)-CHOH(D)) | ||||||
機能・相同性 | ![]() : / all-trans-retinol dehydrogenase (NAD+) activity / alcohol dehydrogenase / retinol metabolic process / retinoic acid metabolic process / zinc ion binding / cytosol 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() | ||||||
手法 | ![]() | ||||||
![]() | Al-Karadaghi, S. / Cedergren-Zeppezauer, E.S. | ||||||
![]() | ![]() タイトル: Refined crystal structure of liver alcohol dehydrogenase-NADH complex at 1.8 A resolution. 著者: Al-Karadaghi, S. / Cedergren-Zeppezauer, E.S. / Hovmoller, S. #1: ![]() タイトル: Interdomain Motion in Liver Alcohol Dehydrogenase. Structural and Energetic Analysis of the Hinge Bending Mode 著者: Colonna-Cesari, F. / Perahia, D. / Karplus, M. / Eklund, H. / Branden, C.I. / Tapia, O. #2: ![]() タイトル: Crystallographic Investigations of Nicotinamide Adenine Dinucleotide Binding to Horse Liver Alcohol Dehydrogenase 著者: Eklund, H. / Samama, J.-P. / Jones, T.A. #3: ![]() タイトル: Crystal Structures of the Active Site in Specifically Metal-Depleted and Cobalt-Substituted Horse Liver Alcohol Dehydrogenase Derivatives 著者: Schneider, G. / Eklund, H. / Cedergren-Zeppezauer, E. / Zeppezauer, M. #4: ![]() タイトル: Three-Dimensional Structure of Isonicotinimidylated Liver Alcohol Dehydrogenase 著者: Plapp, B.V. / Eklund, H. / Jones, T.A. / Branden, C.-I. #5: ![]() タイトル: Crystal-Structure Determination of Reduced Nicotinamide Adenine Dinucleotide Complex with Horse Liver Alcohol Dehydrogenase Maintained in its Apo Conformation by Zinc-Bound Imidazole 著者: Cedergren-Zeppezauer, E. #6: ![]() タイトル: Binding of Substrate in a Ternary Complex of Horse Liver Alcohol Dehydrogenase 著者: Eklund, H. / Plapp, B.V. / Samama, J.-P. / Branden, C.-I. #7: ![]() タイトル: Crystal Structure Determinations of Coenzyme Analogue and Substrate Complexes of Liver Alcohol Dehydrogenase. Binding of 1,4,5,6-Tetrahydronicotinamide Adenine Dinucleotide and Trans-4- ...タイトル: Crystal Structure Determinations of Coenzyme Analogue and Substrate Complexes of Liver Alcohol Dehydrogenase. Binding of 1,4,5,6-Tetrahydronicotinamide Adenine Dinucleotide and Trans-4-(N,N-Dimethylamino)Cinnamaldehyde to the Enzyme 著者: Cedergren-Zeppezauer, E. / Samama, J.-P. / Eklund, H. #8: ![]() タイトル: Pyrazole Binding in Crystalline Binary and Ternary Complexes with Liver Alcohol Dehydrogenase 著者: Eklund, H. / Samama, J.-P. / Wallen, L. #9: ![]() タイトル: 5-Methylnicotinamide-Adenine Dinucleotide. Kinetic Investigation with Major and Minor Isoenzymes of Liver Alcohol Dehydrogenase and Structural Determination of its Binary Complex with Alcohol Dehydrogenase 著者: Samama, J.-P. / Wrixon, A.D. / Biellmann, J.-F. #10: ![]() タイトル: Structure of Triclinic Ternary Complex of Horse Liver Alcohol Dehydrogenase at 2.9 Angstroms Resolution 著者: Eklund, H. / Samama, J.-P. / Wallen, L. / Branden, C.-I. / Akeson, A. / Jones, T.A. #11: ![]() タイトル: Structural Differences between Apo-and Holoenzyme of Horse Liver Alcohol Dehydrogenase 著者: Eklund, H. / Branden, C.-I. #12: ![]() タイトル: X-Ray Studies of the Binding of Cibacron Blue F3Ga to Liver Alcohol Dehydrogenase 著者: Biellmann, J.-F. / Samama, J.-P. / Branden, C.I. / Eklund, H. #13: ![]() タイトル: Crystallography of Liver Alcohol Dehydrogenase Complexed with Substrates 著者: Plapp, B.V. / Eklund, H. / Branden, C.-I. #14: ![]() タイトル: Crystallization of Liver Alcohol Dehydrogenase Activated by the Modification of Amino Groups 著者: Plapp, B.V. / Zeppezauer, E. / Branden, C.-I. #15: ![]() タイトル: Subunit Conformation of Yeast Alcohol Dehydrogenase 著者: Jornvall, H. / Eklund, H. / Branden, C.-I. #16: ![]() タイトル: The Crystal Structure of Complexes between Horse Liver Alcohol Dehydrogenase and the Coenzyme Analogues 3-Iodopyridine-Adenine Dinucleotide and Pyridine-Adenine Dinucleotide 著者: Samama, J.-P. / Zeppezauer, E. / Biellmann, J.-F. / Branden, C.-I. #17: ![]() タイトル: X-Ray Investigation of the Binding of 1,10-Phenanthroline and Imidazole to Horse-Liver Alcohol Dehydrogenase 著者: Boiwe, T. / Branden, C.-I. #18: ![]() タイトル: Three-Dimensional Structure of Horse Liver Alcohol Dehydrogenase at 2.4 Angstroms Resolution 著者: Eklund, H. / Nordstrom, B. / Zeppezauer, E. / Soderlund, G. / Ohlsson, I. / Boiwe, T. / Soderberg, B.-O. / Tapia, O. / Branden, C.-I. / Akeson, A. #19: ![]() タイトル: Structural Comparisons of Mammalian, Yeast and Bacillar Alcohol Dehydrogenases 著者: Eklund, H. / Branden, C.-I. / Jornvall, H. #20: ![]() 年: 1975 タイトル: The Binding of Nucleotides to Horse Liver Alcohol Dehydrogenase 著者: Nordstrom, B. / Branden, C.-I. #21: ![]() タイトル: Alcohol Dehydrogenases 著者: Branden, C.-I. / Jornvall, H. / Eklund, H. / Furugren, B. #22: ![]() タイトル: The Conformation of Adenosine Diphosphoribose and 8-Bromoadenosine Diphosphoribose When Bound to Liver Alcohol Dehydrogenase 著者: Abdallah, M.A. / Biellmann, J.-F. / Nordstrom, B. / Branden, C.-I. #23: ![]() タイトル: The Structure of Horse Liver Alcohol Dehydrogenase 著者: Eklund, H. / Nordstrom, B. / Zeppezauer, E. / Soderlund, G. / Ohlsson, I. / Boiwe, T. / Branden, C.-I. #24: ![]() タイトル: Structural and Functional Similarities within the Coenzyme Binding Domains of Dehydrogenases 著者: Ohlsson, I. / Nordstrom, B. / Branden, C.-I. #25: ![]() タイトル: Binding of Salicylate in the Adenosine-Binding Pocket of Dehydrogenases 著者: Einarsson, R. / Eklund, H. / Zeppezauer, E. / Boiwe, T. / Branden, C.-I. #26: ![]() タイトル: Structure of Liver Alcohol Dehydrogenase at 2.9-Angstroms Resolution 著者: Branden, C.-I. / Eklund, H. / Nordstrom, B. / Boiwe, T. / Soderlund, G. / Zeppezauer, E. / Ohlsson, I. / Akeson, A. #27: ![]() タイトル: Structure of Horse Liver Alcohol Dehydrogenase. I. Structural Symmetry and Conformational Changes 著者: Branden, C.-I. | ||||||
履歴 |
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Remark 650 | HELIX GLY 181 - VAL 189 IS A PI-HELIX (CLASS 3, 1.5 TURNS) AT HELIX IDENTIFICATION HAA AND HAB. |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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PDBx/mmCIF形式 | ![]() | 166 KB | 表示 | ![]() |
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PDB形式 | ![]() | 129.5 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
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集合体
登録構造単位 | ![]()
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単位格子 |
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Atom site foot note | 1: RESIDUES PRO A 62 AND PRO B 62 ARE CIS PROLINES. 2: THE FOLLOWING RESIDUES WERE DISCRETELY DISORDERED: CYS A 281, CYS A 282, AND CYS B 282 | ||||||||
非結晶学的対称性 (NCS) | NCS oper: (Code: given Matrix: (0.12925, 0.98584, -0.10681), ベクター: |
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要素
#1: タンパク質 | 分子量: 39853.273 Da / 分子数: 2 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() #2: 化合物 | ChemComp-ZN / #3: 化合物 | #4: 化合物 | #5: 水 | ChemComp-HOH / | |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.47 Å3/Da / 溶媒含有率: 50.19 % | ||||||||||||||||||||||||||||||||||||||||||
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結晶化 | *PLUS pH: 7 / 手法: microdialysis | ||||||||||||||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
反射 | *PLUS 最高解像度: 1.8 Å / 最低解像度: 10 Å / Num. obs: 62440 / % possible obs: 88.5 % / Rmerge(I) obs: 0.08 |
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解析
ソフトウェア |
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精密化 | 解像度: 1.8→10 Å / Rfactor Rwork: 0.173 / Rfactor obs: 0.173 / σ(F): 0 詳細: THE FOLLOWING RESIDUES WERE DISCRETELY DISORDERED: CYS A 281, CYS A 282, AND CYS B 282 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 1.8→10 Å
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拘束条件 |
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