Autophagy-RelatedProteinLC3C / Autophagy-related protein LC3 C / Autophagy-related ubiquitin-like modifier LC3 C / MAP1 light ...Autophagy-related protein LC3 C / Autophagy-related ubiquitin-like modifier LC3 C / MAP1 light chain 3-like protein 3 / MAP1A/MAP1B light chain 3 C / MAP1A/MAP1B LC3 C / Microtubule-associated protein 1 light chain 3 gamma
Mass: 14802.249 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: MAP1LC3C / Production host: Escherichia coli (E. coli) / References: UniProt: Q9BXW4
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
3
2D 1H-1H TOCSY
1
2
3
2D 1H-1H NOESY
1
3
1
2D 1H-15N HSQC
1
4
1
3D 1H-15N TOCSY
1
5
1
3D 1H-15N NOESY
1
6
1
3D 1H-15N 1H-15N NOESY
1
7
2
3D HNHA
1
8
3
2D 1H-13C HSQC
1
9
2
3D HNCO
1
10
2
3D HNCA
1
11
2
3D HN(CA)CB
1
12
2
3DCBCA(CO)NH
1
13
2
3DHBHA(CO)NH
1
14
2
3DC(CO)NH
1
15
2
3DH(CCO)NH
1
16
3
3D (H)CCH-COSY
1
17
3
3D (H)CCH-COSY
1
18
3
3D (H)CCH-TOCSY
1
19
3
3D 1H-13C NOESY
1
20
2
3D 1H-13C 1H-15N NOESY
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Sample preparation
Details
Solution-ID
Contents
Solvent system
1
0.35-0.7 mM [U-15N] Microtubule-associated protein light chain 3C (LC3C), 20 mM PIPES, 150 mM sodium chloride, 0.1 mM EDTA, 2% [U-2H] glycerol, 90% H2O, 10% D2O, 90% H2O/10% D2O
90% H2O/10% D2O
2
0.35-0.7 mM [U-13C; U-15N] Microtubule-associated protein light chain 3C (LC3C), 20 mM PIPES, 150 mM sodium chloride, 0.1 mM EDTA, 2% [U-2H] glycerol, 90% H2O, 10% D2O, 90% H2O/10% D2O
90% H2O/10% D2O
3
0.35-0.7 mM [U-13C; U-15N] Microtubule-associated protein light chain 3C (LC3C), 20 mM PIPES, 150 mM sodium chloride, 0.1 mM EDTA, 2% [U-2H] glycerol, 100% D2O, 100% D2O
100% D2O
Sample
Conc. (mg/ml)
Units
Component
Isotopic labeling
Conc. range (mg/ml)
Solution-ID
mM
Microtubule-associated protein light chain 3C (LC3C)-1
[U-15N]
0.35-0.7
1
20mM
PIPES-2
1
150mM
sodium chloride-3
1
0.1mM
EDTA-4
1
2 %
glycerol-5
[U-2H]
1
90 %
H2O-6
1
10 %
D2O-7
1
mM
Microtubule-associated protein light chain 3C (LC3C)-8
[U-13C; U-15N]
0.35-0.7
2
20mM
PIPES-9
2
150mM
sodium chloride-10
2
0.1mM
EDTA-11
2
2 %
glycerol-12
[U-2H]
2
90 %
H2O-13
2
10 %
D2O-14
2
mM
Microtubule-associated protein light chain 3C (LC3C)-15
NOE constraints total: 1162 / NOE intraresidue total count: 202 / NOE long range total count: 307 / NOE medium range total count: 266 / NOE sequential total count: 387 / Hydrogen bond constraints total count: 102 / Protein chi angle constraints total count: 0 / Protein other angle constraints total count: 0 / Protein phi angle constraints total count: 123 / Protein psi angle constraints total count: 91
NMR representative
Selection criteria: lowest energy
NMR ensemble
Average torsion angle constraint violation: 0.129 ° Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 10 / Maximum lower distance constraint violation: 0.25 Å / Maximum torsion angle constraint violation: 3.77 ° / Maximum upper distance constraint violation: 0.34 Å / Torsion angle constraint violation method: CING
NMR ensemble rms
Distance rms dev: 0.017 Å / Distance rms dev error: 0.002 Å
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