Journal: Rna / Year: 2015 Title: The NMR structure of the II-III-VI three-way junction from the Neurospora VS ribozyme reveals a critical tertiary interaction and provides new insights into the global ribozyme structure. Authors: Bonneau, E. / Girard, N. / Lemieux, S. / Legault, P.
Mass: 20021.980 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: RNA was prepared by in vitro transcription with T7 RNA polymerase Source: (synth.) synthetic construct (others)
-
Experimental details
-
Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
1D 1H WATERGATE
1
2
1
2D 1H-15N HSQC
1
3
1
2D 1H-15N HSQC NH2 only
1
4
1
2D HNN COSY
1
5
1
2D 1H-1H NOESY
1
6
1
2D 1H-15N CPMG-NOESY
1
7
1
3D 15N-edited NOESY-HSQC
1
8
6
3D 15N/13C-edited NOESY-HSQC
1
9
6
2D G-specific H(NC)-TOCSY-(C)H
1
10
6
2D A-specific H(NC)-TOCSY-(C)H
2
11
2
2D 1H-15N MQ-(HC)N(C)H
2
12
2
2D 1H-13C HSQC
2
13
2
2D 1H-13C HMQC
2
14
2
3D (H)CCH-COSY
2
15
2
3D (H)CCH-TOCSY
2
16
2
3D 13C-edited NOESY-HSQC
2
17
3
2D 1H-13C HSQC
2
18
3
2D 1H-13C HMQC
2
19
3
3D (H)CCH-COSY
2
20
3
3D (H)CCH-TOCSY
2
21
3
3D 13C-edited NOESY-HSQC
2
22
4
2D 1H-13C HSQC
2
23
4
2D 1H-13C HMQC
2
24
4
3D (H)CCH-COSY
2
25
4
3D (H)CCH-TOCSY
2
26
4
3D 13C-edited NOESY-HSQC
2
27
5
2D 1H-13C HSQC
2
28
5
2D 1H-13C HMQC
2
29
5
3D (H)CCH-COSY
2
30
5
3D (H)CCH-TOCSY
2
31
5
3D 13C-edited NOESY-HSQC
-
Sample preparation
Details
Solution-ID
Contents
Solvent system
1
2 mM [U-15N] RNA (62-MER), 5mM MgCl2, 90% H2O/10% D2O
90% H2O/10% D2O
2
2 mM [U-13C; U-15N] RNA (62-MER), 5mM MgCl2, 100% D2O
100% D2O
3
2 mM [U-13C; U-15N]-Ade RNA (62-MER), 5mM MgCl2, 100% D2O
100% D2O
4
2 mM [U-13C; U-15N]-Cyt RNA (62-MER), 5mM MgCl2, 100% D2O
100% D2O
5
2.5 mM [U-13C; U-15N]-Gua RNA (62-MER), 5mM MgCl2, 100% D2O
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