[English] 日本語
Yorodumi- PDB-2mqj: Solution structure of ubiquitin-like protein from Caldiarchaeum s... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2mqj | ||||||
---|---|---|---|---|---|---|---|
Title | Solution structure of ubiquitin-like protein from Caldiarchaeum subterraneum | ||||||
Components | Ubiquitin-like protein | ||||||
Keywords | LIGASE / Caldiarchaeum subterraneum | ||||||
Function / homology | Function and homology information polyubiquitin modification-dependent protein binding / ubiquitin-dependent protein catabolic process / metal ion binding / cytosol Similarity search - Function | ||||||
Biological species | Candidatus Caldiarchaeum subterraneum (archaea) | ||||||
Method | SOLUTION NMR / distance geometry | ||||||
Model details | lowest energy, model1 | ||||||
Authors | Ye, K. | ||||||
Citation | Journal: To be Published Title: Solution structure of ubiquitin-like protein from Caldiarchaeum subterraneum Authors: Ye, K. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 2mqj.cif.gz | 530.2 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb2mqj.ent.gz | 449.6 KB | Display | PDB format |
PDBx/mmJSON format | 2mqj.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2mqj_validation.pdf.gz | 542.4 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 2mqj_full_validation.pdf.gz | 752 KB | Display | |
Data in XML | 2mqj_validation.xml.gz | 36.5 KB | Display | |
Data in CIF | 2mqj_validation.cif.gz | 51.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mq/2mqj ftp://data.pdbj.org/pub/pdb/validation_reports/mq/2mqj | HTTPS FTP |
-Related structure data
Similar structure data | |
---|---|
Other databases |
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 9258.878 Da / Num. of mol.: 1 / Fragment: UNP residues 1-77 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Candidatus Caldiarchaeum subterraneum (archaea) Gene: CSUB_C1474, HGMM_F04B03C03, HGMM_F21D07C21, HGMM_F30C12C33 Production host: Escherichia coli (E. coli) / References: UniProt: E6N8B8 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-Sample preparation
Details | Contents: 1mM [U-100% 13C; U-100% 15N] sodium phosphate-1, 1mM [U-100% 15N] sodium phosphate-2, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample |
| ||||||||||||
Sample conditions | Ionic strength: 0.1 / pH: 6.8 / Pressure: ambient / Temperature: 293 K |
-NMR measurement
NMR spectrometer | Type: Bruker DMX / Manufacturer: Bruker / Model: DMX / Field strength: 500 MHz |
---|
-Processing
NMR software |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: distance geometry / Software ordinal: 1 | |||||||||
NMR representative | Selection criteria: lowest energy | |||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 / Representative conformer: 1 |