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- PDB-2mqj: Solution structure of ubiquitin-like protein from Caldiarchaeum s... -
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Basic information
Entry | Database: PDB / ID: 2mqj | ||||||
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Title | Solution structure of ubiquitin-like protein from Caldiarchaeum subterraneum | ||||||
![]() | Ubiquitin-like protein | ||||||
![]() | LIGASE / Caldiarchaeum subterraneum | ||||||
Function / homology | ![]() polyubiquitin modification-dependent protein binding / ubiquitin-dependent protein catabolic process / metal ion binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / distance geometry | ||||||
Model details | lowest energy, model1 | ||||||
![]() | Ye, K. | ||||||
![]() | ![]() Title: Solution structure of ubiquitin-like protein from Caldiarchaeum subterraneum Authors: Ye, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 530.2 KB | Display | ![]() |
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PDB format | ![]() | 449.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 542.4 KB | Display | ![]() |
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Full document | ![]() | 752 KB | Display | |
Data in XML | ![]() | 36.5 KB | Display | |
Data in CIF | ![]() | 51.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 9258.878 Da / Num. of mol.: 1 / Fragment: UNP residues 1-77 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: CSUB_C1474, HGMM_F04B03C03, HGMM_F21D07C21, HGMM_F30C12C33 Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Contents: 1mM [U-100% 13C; U-100% 15N] sodium phosphate-1, 1mM [U-100% 15N] sodium phosphate-2, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O | ||||||||||||
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Sample |
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Sample conditions | Ionic strength: 0.1 / pH: 6.8 / Pressure: ambient / Temperature: 293 K |
-NMR measurement
NMR spectrometer | Type: Bruker DMX / Manufacturer: Bruker / Model: DMX / Field strength: 500 MHz |
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Processing
NMR software |
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Refinement | Method: distance geometry / Software ordinal: 1 | |||||||||
NMR representative | Selection criteria: lowest energy | |||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 / Representative conformer: 1 |