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- PDB-2lp5: Native Structure of the Fyn SH3 A39V/N53P/V55L -

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Basic information

Entry
Database: PDB / ID: 2lp5
TitleNative Structure of the Fyn SH3 A39V/N53P/V55L
ComponentsTyrosine-protein kinase Fyn
KeywordsTRANSFERASE / amyloid fibril / protein folding intermediate
Function / homology
Function and homology information


reelin-mediated signaling pathway / forebrain development / tubulin binding / cell surface receptor protein tyrosine kinase signaling pathway / peptidyl-tyrosine phosphorylation / non-membrane spanning protein tyrosine kinase activity / non-specific protein-tyrosine kinase / neuron migration / T cell receptor signaling pathway / regulation of cell shape ...reelin-mediated signaling pathway / forebrain development / tubulin binding / cell surface receptor protein tyrosine kinase signaling pathway / peptidyl-tyrosine phosphorylation / non-membrane spanning protein tyrosine kinase activity / non-specific protein-tyrosine kinase / neuron migration / T cell receptor signaling pathway / regulation of cell shape / protein tyrosine kinase activity / perikaryon / cell differentiation / protein kinase activity / membrane raft / signaling receptor binding / ATP binding / metal ion binding / nucleus / plasma membrane / cytosol
Similarity search - Function
Fyn/Yrk, SH3 domain / SH3 Domains / : / SH3 domain / SH2 domain / Src homology 2 (SH2) domain profile. / Src homology 2 domains / SH2 domain / SH3 type barrels. / Src homology 3 domains ...Fyn/Yrk, SH3 domain / SH3 Domains / : / SH3 domain / SH2 domain / Src homology 2 (SH2) domain profile. / Src homology 2 domains / SH2 domain / SH3 type barrels. / Src homology 3 domains / SH2 domain superfamily / SH3-like domain superfamily / Src homology 3 (SH3) domain profile. / SH3 domain / Tyrosine-protein kinase, catalytic domain / Tyrosine kinase, catalytic domain / Tyrosine protein kinases specific active-site signature. / Tyrosine-protein kinase, active site / Serine-threonine/tyrosine-protein kinase, catalytic domain / Protein tyrosine and serine/threonine kinase / Roll / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily / Mainly Beta
Similarity search - Domain/homology
Tyrosine-protein kinase Fyn
Similarity search - Component
Biological speciesGallus gallus (chicken)
MethodSOLUTION NMR / simulated annealing
Model detailslowest energy, model 1
AuthorsNeudecker, P. / Robustelli, P. / Cavalli, A. / Vendruscolo, M. / Kay, L.E.
CitationJournal: Science / Year: 2012
Title: Structure of an intermediate state in protein folding and aggregation.
Authors: Neudecker, P. / Robustelli, P. / Cavalli, A. / Walsh, P. / Lundstrom, P. / Zarrine-Afsar, A. / Sharpe, S. / Vendruscolo, M. / Kay, L.E.
History
DepositionFeb 6, 2012Deposition site: BMRB / Processing site: RCSB
Revision 1.0May 16, 2012Provider: repository / Type: Initial release
Revision 1.1Jun 14, 2023Group: Advisory / Data collection ...Advisory / Data collection / Database references / Other
Category: database_2 / pdbx_database_remark ...database_2 / pdbx_database_remark / pdbx_database_status / pdbx_nmr_software / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_remark.text / _pdbx_database_status.status_code_nmr_data / _pdbx_nmr_software.name / _struct_ref_seq_dif.details
Revision 1.2May 15, 2024Group: Data collection / Database references / Category: chem_comp_atom / chem_comp_bond / database_2 / Item: _database_2.pdbx_DOI
Remark 650HELIX DETERMINATION METHOD: AUTHOR DETERMINED
Remark 700SHEET DETERMINATION METHOD: AUTHOR DETERMINED

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Tyrosine-protein kinase Fyn


Theoretical massNumber of molelcules
Total (without water)7,5381
Polymers7,5381
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)10 / 1000structures with the lowest energy
RepresentativeModel #1lowest energy

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Components

#1: Protein Tyrosine-protein kinase Fyn / Proto-oncogene c-Fyn / p59-Fyn


Mass: 7538.242 Da / Num. of mol.: 1 / Fragment: SH3 domain residues 85-142 / Mutation: A39V, N53P, V55L
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Gallus gallus (chicken) / Gene: FYN / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)
References: UniProt: Q05876, non-specific protein-tyrosine kinase

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
Details: Solution structure of the native Fyn SH3 domain mutant A39V/N53P/V55L from G. gallus
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
1122D 1H-15N HSQC
1213D 1H-15N NOESY-HSQC
1373D HNCO
1473D HNCA
1573D C(CO)NH
1682D 1H-13C CTHSQC
17102D 1H-13C HSQC
18215N SQ CPMG
19215N SQ CPMG
110215N TROSY/AntiTROSY CPMG
111215N TROSY/AntiTROSY CPMG
112315N SQ CPMG
113315N SQ CPMG
114315N TROSY/AntiTROSY CPMG
115315N TROSY/AntiTROSY CPMG
116415N SQ CPMG
117415N SQ CPMG
118415N TROSY/AntiTROSY CPMG
119415N TROSY/AntiTROSY CPMG
12021HN SQ CPMG
12121HN SQ CPMG
122715N SQ CPMG
123713CO SQ CPMG
124713CO SQ CPMG
125515N SQ CPMG
126513CO SQ CPMG
127513CO SQ CPMG
128615N SQ CPMG
129613CO SQ CPMG
130613CO SQ CPMG
131913CA SQ CPMG
132913CA SQ CPMG
1331113CA SQ CPMG
1341113CA SQ CPMG
13581HA SQ CPMG
13681HA SQ CPMG
1371013CH3 SQ CPMG
1381013CH3 SQ CPMG
NMR detailsText: The CPMG NMR relaxation dispersion experiments were supplemented with sign determination experiments as appropriate.

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Sample preparation

Details
Solution-IDContentsSolvent system
10.7 mM [U-15N] Fyn SH3 A39V/N53P/V55L, 0.2 mM EDTA, 0.05 % sodium azide, 50 mM potassium phosphate, 90% H2O/10% D2O90% H2O/10% D2O
21.0 mM [U-15N; U-2H] Fyn SH3 A39V/N53P/V55L, 0.2 mM EDTA, 0.05 % sodium azide, 50 mM potassium phosphate, 90% H2O/10% D2O90% H2O/10% D2O
30.8 mM [U-15N; U-2H] Fyn SH3 A39V/N53P/V55L, 0.2 mM EDTA, 0.05 % sodium azide, 50 mM potassium phosphate, 24 mg/ml Pf1 phage, 90% H2O/10% D2O90% H2O/10% D2O
40.5 mM [U-15N; U-2H] Fyn SH3 A39V/N53P/V55L, 0.2 mM EDTA, 0.05 % sodium azide, 50 mM potassium phosphate, uncertain v/v PEG/hexanol, 90% H2O/10% D2O90% H2O/10% D2O
51.2 mM [U-13C; U-15N; U-2H] Fyn SH3 A39V/N53P/V55L, 0.2 mM EDTA, 0.05 % sodium azide, 50 mM potassium phosphate, 90% H2O/10% D2O90% H2O/10% D2O
60.8 mM [U-13C; U-15N; U-2H] Fyn SH3 A39V/N53P/V55L, 0.2 mM EDTA, 0.05 % sodium azide, 50 mM potassium phosphate, 36 mg/ml Pf1 phage, 90% H2O/10% D2O90% H2O/10% D2O
71.0 mM [U-13C; U-15N; U-50% 2H] Fyn SH3 A39V/N53P/V55L, 0.2 mM EDTA, 0.05 % sodium azide, 50 mM potassium phosphate, 90% H2O/10% D2O90% H2O/10% D2O
81.0 mM [U-13C; U-15N; U-50% 2H] Fyn SH3 A39V/N53P/V55L, 0.2 mM EDTA, 0.05 % sodium azide, 50 mM potassium phosphate, 100% D2O100% D2O
91.0 mM [2'-13C glucose; U-15N] Fyn SH3 A39V/N53P/V55L, 0.2 mM EDTA, 0.05 % sodium azide, 50 mM potassium phosphate, 100% D2O100% D2O
101.0 mM [1'-13C glucose; U-15N] Fyn SH3 A39V/N53P/V55L, 0.2 mM EDTA, 0.05 % sodium azide, 50 mM potassium phosphate, 90% H2O/10% D2O90% H2O/10% D2O
111.2 mM [1'-13C glucose; U-15N] Fyn SH3 A39V/N53P/V55L, 0.2 mM EDTA, 0.05 % sodium azide, 50 mM potassium phosphate, 100% D2O100% D2O
Sample
Conc. (mg/ml)UnitsComponentIsotopic labelingSolution-ID
0.7 mMFyn SH3 A39V/N53P/V55L-1[U-15N]1
0.2 mMEDTA-21
0.05 %sodium azide-31
50 mMpotassium phosphate-41
90 %H2O-51
10 %D2O-61
1.0 mMFyn SH3 A39V/N53P/V55L-7[U-15N; U-2H]2
0.2 mMEDTA-82
0.05 %sodium azide-92
50 mMpotassium phosphate-102
90 %H2O-112
10 %D2O-122
0.8 mMFyn SH3 A39V/N53P/V55L-13[U-15N; U-2H]3
0.2 mMEDTA-143
0.05 %sodium azide-153
50 mMpotassium phosphate-163
24 mg/mLPf1 phage-173
90 %H2O-183
10 %D2O-193
0.5 mMFyn SH3 A39V/N53P/V55L-20[U-15N; U-2H]4
0.2 mMEDTA-214
0.05 %sodium azide-224
50 mMpotassium phosphate-234
v/vPEG/hexanol-244
90 %H2O-254
10 %D2O-264
1.2 mMFyn SH3 A39V/N53P/V55L-27[U-13C; U-15N; U-2H]5
0.2 mMEDTA-285
0.05 %sodium azide-295
50 mMpotassium phosphate-305
90 %H2O-315
10 %D2O-325
0.8 mMFyn SH3 A39V/N53P/V55L-33[U-13C; U-15N; U-2H]6
0.2 mMEDTA-346
0.05 %sodium azide-356
50 mMpotassium phosphate-366
36 mg/mLPf1 phage-376
90 %H2O-386
10 %D2O-396
1.0 mMFyn SH3 A39V/N53P/V55L-40[U-13C; U-15N; U-50% 2H]7
0.2 mMEDTA-417
0.05 %sodium azide-427
50 mMpotassium phosphate-437
90 %H2O-447
10 %D2O-457
1.0 mMFyn SH3 A39V/N53P/V55L-46[U-13C; U-15N; U-50% 2H]8
0.2 mMEDTA-478
0.05 %sodium azide-488
50 mMpotassium phosphate-498
100 %D2O-508
1.0 mMFyn SH3 A39V/N53P/V55L-51[2'-13C glucose; U-15N]9
0.2 mMEDTA-529
0.05 %sodium azide-539
50 mMpotassium phosphate-549
100 %D2O-559
1.0 mMFyn SH3 A39V/N53P/V55L-56[1'-13C glucose; U-15N]10
0.2 mMEDTA-5710
0.05 %sodium azide-5810
50 mMpotassium phosphate-5910
90 %H2O-6010
10 %D2O-6110
1.2 mMFyn SH3 A39V/N53P/V55L-62[1'-13C glucose; U-15N]11
0.2 mMEDTA-6311
0.05 %sodium azide-6411
50 mMpotassium phosphate-6511
10 %D2O-6611
Sample conditionsIonic strength: 50 / pH: 7 / Pressure: ambient / Temperature: 293.15 K

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NMR measurement

NMR spectrometer
TypeManufacturerModelField strength (MHz)Spectrometer-ID
Varian INOVAVarianINOVA8001
Varian INOVAVarianINOVA6002
Varian INOVAVarianINOVA5003
Varian INOVAVarianINOVA5004

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Processing

NMR software
NameVersionDeveloperClassification
NMRPipe3.0 Rev 2007.068.09.07Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Baxprocessing
NMRViewJ8.0.rc4Johnson, One Moon Scientificspectra analysis
MUNIN1Orekhov, Ibraghimov, Billeterpeak picking
CATIA0.52pHansencpmg analysis
ALMOST2Cavallistructure solution
TALOS+1.01FShen, Delaglio, Cornilescu, Baxbackbone torsion dihedral angle restraints
ALMOST2Cavallirefinement
RefinementMethod: simulated annealing / Software ordinal: 1
Details: Torsion angle molecular dynamics simulated annealing refinement of an initial model based on the crystal structure of the Fyn SH3 domain mutant N53I/V55L (PDB 3CQT) with NOE distance, ...Details: Torsion angle molecular dynamics simulated annealing refinement of an initial model based on the crystal structure of the Fyn SH3 domain mutant N53I/V55L (PDB 3CQT) with NOE distance, chemical shift derived backbone torsion dihedral, and RDC restraints
NMR constraintsNOE constraints total: 416 / NOE intraresidue total count: 116 / NOE long range total count: 82 / NOE medium range total count: 42 / NOE sequential total count: 176 / Protein phi angle constraints total count: 35 / Protein psi angle constraints total count: 38
NMR representativeSelection criteria: lowest energy
NMR ensembleConformer selection criteria: structures with the lowest energy
Conformers calculated total number: 1000 / Conformers submitted total number: 10

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