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- PDB-2lew: Structural Plasticity of Paneth cell alpha-Defensins: Characteriz... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2lew | ||||||
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Title | Structural Plasticity of Paneth cell alpha-Defensins: Characterization of Salt-Bridge Deficient Analogues of Mouse Cryptdin-4 | ||||||
![]() | Alpha-defensin 4![]() | ||||||
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Function / homology | ![]() pore-forming activity / disruption of plasma membrane integrity in another organism / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
Model details | lowest energy, model 1 | ||||||
![]() | Rosengren, K. / Andersson, H.S. / Haugaard-Kedstrom, L.M. / Bengtsson, E. / Daly, N.L. / Craik, D.J. | ||||||
![]() | ![]() Title: The alpha-defensin salt-bridge induces backbone stability to facilitate folding and confer proteolytic resistance. Authors: Andersson, H.S. / Figueredo, S.M. / Haugaard-Kedstrom, L.M. / Bengtsson, E. / Daly, N.L. / Qu, X. / Craik, D.J. / Ouellette, A.J. / Rosengren, K.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 200.4 KB | Display | ![]() |
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PDB format | ![]() | 173.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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NMR ensembles |
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Components
#1: Protein/peptide | ![]() Mass: 3699.547 Da / Num. of mol.: 1 / Mutation: E15G Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() | ||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Contents: 0.3 mg (E15G)-Crp4, 90% H2O/10% D2O / Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 0.3 mg/mL / Component: (E15G)-Crp4-1 |
Sample conditions | pH: 4 / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: ![]() Details: Torsion angle dynamics followed by refinement by Cartesian dynamics in explicit water. | |||||||||
NMR representative | Selection criteria: lowest energy | |||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 50 / Conformers submitted total number: 20 |