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Yorodumi- PDB-2kg4: Three-dimensional structure of human Gadd45alpha in solution by NMR -
+Open data
-Basic information
Entry | Database: PDB / ID: 2kg4 | ||||||
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Title | Three-dimensional structure of human Gadd45alpha in solution by NMR | ||||||
Components | Growth arrest and DNA-damage-inducible protein GADD45 alpha | ||||||
Keywords | CELL CYCLE / gadd45 / growth arrest / DNA damage / flexible regions / monomer | ||||||
Function / homology | Function and homology information negative regulation of peptidyl-serine phosphorylation of STAT protein / regulation of cell cycle => GO:0051726 / : / regulation of cell cycle => GO:0051726 / FOXO-mediated transcription of cell cycle genes / : / negative regulation of protein serine/threonine kinase activity / negative regulation of blood vessel endothelial cell migration / centrosome cycle / positive regulation of p38MAPK cascade ...negative regulation of peptidyl-serine phosphorylation of STAT protein / regulation of cell cycle => GO:0051726 / : / regulation of cell cycle => GO:0051726 / FOXO-mediated transcription of cell cycle genes / : / negative regulation of protein serine/threonine kinase activity / negative regulation of blood vessel endothelial cell migration / centrosome cycle / positive regulation of p38MAPK cascade / regulation of cyclin-dependent protein serine/threonine kinase activity / signal transduction in response to DNA damage / negative regulation of angiogenesis / DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest / TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest / promoter-specific chromatin binding / cellular response to ionizing radiation / positive regulation of JNK cascade / kinase binding / cellular response to mechanical stimulus / positive regulation of reactive oxygen species metabolic process / regulation of cell cycle / nuclear speck / protein heterodimerization activity / positive regulation of apoptotic process / DNA repair / apoptotic process / negative regulation of transcription by RNA polymerase II / protein homodimerization activity / nucleoplasm / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / molecular dynamics | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Sanchez, R. / Pantoja-Uceda, D. / Prieto, J. / Diercks, T. / Campos-Olivas, R. / Blanco, F.J. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2010 Title: Solution structure of human growth arrest and DNA damage 45alpha (Gadd45alpha) and its interactions with proliferating cell nuclear antigen (PCNA) and Aurora A kinase Authors: Sanchez, R. / Pantoja-Uceda, D. / Prieto, J. / Diercks, T. / Marcaida, M.J. / Montoya, G. / Campos-Olivas, R. / Blanco, F.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2kg4.cif.gz | 986 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2kg4.ent.gz | 827.6 KB | Display | PDB format |
PDBx/mmJSON format | 2kg4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2kg4_validation.pdf.gz | 473.6 KB | Display | wwPDB validaton report |
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Full document | 2kg4_full_validation.pdf.gz | 829.8 KB | Display | |
Data in XML | 2kg4_validation.xml.gz | 118.7 KB | Display | |
Data in CIF | 2kg4_validation.cif.gz | 110.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/kg/2kg4 ftp://data.pdbj.org/pub/pdb/validation_reports/kg/2kg4 | HTTPS FTP |
-Related structure data
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Other databases |
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-Links
-Assembly
Deposited unit |
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NMR ensembles |
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-Components
#1: Protein | Mass: 18351.723 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: GADD45A / Production host: Escherichia coli (E. coli) / Strain (production host): Rosetta (BL21)DE3 / References: UniProt: P24522 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
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