PROTEIN TRANSPORT / VPS4B / MIT / Three Helix Bundle
機能・相同性
機能・相同性情報
protein depolymerization / late endosomal microautophagy / positive regulation of centriole elongation / ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway / negative regulation of exosomal secretion / late endosome to lysosome transport via multivesicular body sorting pathway / ESCRT III complex disassembly / regulation of centrosome duplication / nuclear membrane reassembly / multivesicular body sorting pathway ...protein depolymerization / late endosomal microautophagy / positive regulation of centriole elongation / ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway / negative regulation of exosomal secretion / late endosome to lysosome transport via multivesicular body sorting pathway / ESCRT III complex disassembly / regulation of centrosome duplication / nuclear membrane reassembly / multivesicular body sorting pathway / establishment of blood-brain barrier / vacuole organization / positive regulation of exosomal secretion / midbody abscission / membrane fission / plasma membrane repair / ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway / multivesicular body assembly / endosome to lysosome transport via multivesicular body sorting pathway / cholesterol transport / regulation of mitotic spindle assembly / Flemming body / vesicle-fusing ATPase / endosomal transport / mitotic metaphase chromosome alignment / response to lipid / ATPase complex / nucleus organization / viral budding via host ESCRT complex / autophagosome maturation / canonical Wnt signaling pathway / nuclear pore / positive regulation of G2/M transition of mitotic cell cycle / Endosomal Sorting Complex Required For Transport (ESCRT) / viral budding from plasma membrane / macroautophagy / Budding and maturation of HIV virion / potassium ion transport / autophagy / spindle pole / late endosome membrane / protein transport / midbody / angiogenesis / endosome / endosome membrane / centrosome / protein-containing complex binding / protein homodimerization activity / ATP hydrolysis activity / extracellular exosome / ATP binding / identical protein binding / nucleus / plasma membrane / cytosol / cytoplasm 類似検索 - 分子機能
Vacuolar protein sorting-associated protein 4, MIT domain / Phosphotransferase system, lactose/cellobiose-type IIA subunit / MIT (microtubule interacting and transport) domain / MIT domain superfamily / Vps4 oligomerisation, C-terminal / MIT domain / Microtubule Interacting and Trafficking molecule domain / : / Vps4 C terminal oligomerisation domain / AAA ATPase, AAA+ lid domain ...Vacuolar protein sorting-associated protein 4, MIT domain / Phosphotransferase system, lactose/cellobiose-type IIA subunit / MIT (microtubule interacting and transport) domain / MIT domain superfamily / Vps4 oligomerisation, C-terminal / MIT domain / Microtubule Interacting and Trafficking molecule domain / : / Vps4 C terminal oligomerisation domain / AAA ATPase, AAA+ lid domain / AAA+ lid domain / ATPase, AAA-type, conserved site / AAA-protein family signature. / Methane Monooxygenase Hydroxylase; Chain G, domain 1 / ATPase family associated with various cellular activities (AAA) / ATPase, AAA-type, core / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / Up-down Bundle / P-loop containing nucleoside triphosphate hydrolase / Mainly Alpha 類似検索 - ドメイン・相同性
Vacuolar protein sorting-associated protein 4B 類似検索 - 構成要素
濃度: 1.8 mM / 構成要素: VPS4B MIT / Isotopic labeling: [U-100% 13C; U-100% 15N]
試料状態
イオン強度: 50 / pH: 5.65 / 圧: ambient / 温度: 298 K
-
NMR測定
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Varian INOVA
Varian
INOVA
600
1
Varian INOVA
Varian
INOVA
800
2
-
解析
NMR software
名称
バージョン
開発者
分類
Felix
2004
AccelrysSoftwareInc.
解析
Sparky
3.112
Goddard
chemicalshiftassignment
Sparky
3.112
Goddard
peakpicking
Sparky
3.112
Goddard
データ解析
CYANA
2.1
Guntert, MumenthalerandWuthrich
構造決定
CNS
1.2
Brunger, Adams, Clore, Gros, NilgesandRead
精密化
精密化
手法: simulated annealing / ソフトェア番号: 1 詳細: Refinement using anneal.inp and using summed NOE constraints with max T of 3000 K for simulated annealing.
NMR constraints
NOE constraints total: 1769 / NOE intraresidue total count: 426 / NOE long range total count: 387 / NOE medium range total count: 538 / NOE sequential total count: 418 / Hydrogen bond constraints total count: 55 / Protein chi angle constraints total count: 0 / Protein other angle constraints total count: 0 / Protein phi angle constraints total count: 60 / Protein psi angle constraints total count: 60
代表構造
選択基準: lowest energy
NMRアンサンブル
Average torsion angle constraint violation: 0.03 ° コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 200 / 登録したコンフォーマーの数: 20 / Maximum lower distance constraint violation: 0 Å / Maximum torsion angle constraint violation: 0.1 ° / Maximum upper distance constraint violation: 0.065 Å / Torsion angle constraint violation method: CNS