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Open data
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Basic information
Entry | Database: PDB / ID: 2j8m | ||||||
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Title | Structure of P. aeruginosa acetyltransferase PA4866 | ||||||
![]() | ACETYLTRANSFERASE PA4866 FROM P. AERUGINOSA | ||||||
![]() | TRANSFERASE / GCN5 FAMILY / PHOSPHINOTHRICIN / METHIONINE SULFONE / TRANSFERASE METHIONINE SULFOXIMINE / N-ACETYL TRANSFERASE / HYPOTHETICAL PROTEIN | ||||||
Function / homology | ![]() acyltransferase activity, transferring groups other than amino-acyl groups / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Davies, A.M. / Tata, R. / Beavil, R.L. / Sutton, B.J. / Brown, P.R. | ||||||
![]() | ![]() Title: L-Methionine Sulfoximine, But not Phosphinothricin, is a Substrate for an Acetyltransferase (Gene Pa4866) from Pseudomonas Aeruginosa: Structural and Functional Studies. Authors: Davies, A.M. / Tata, R. / Beavil, R.L. / Sutton, B.J. / Brown, P.R. #1: ![]() Title: Crystal Structure of a Putative Phosphinothricin Acetyltransferase (Pa4866) from Pseudomonas Aeruginosa Pac1 Authors: Davies, A.M. / Tata, R. / Agha, R. / Sutton, B.J. / Brown, P.R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 95.4 KB | Display | ![]() |
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PDB format | ![]() | 72.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 423.2 KB | Display | ![]() |
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Full document | ![]() | 429.6 KB | Display | |
Data in XML | ![]() | 18.8 KB | Display | |
Data in CIF | ![]() | 30.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2j8nC ![]() 2j8rC ![]() 2bl1S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.86014, 0.50103, 0.09557), Vector: |
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Components
#1: Protein | Mass: 18732.076 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-AZI / #3: Chemical | ChemComp-GOL / #4: Chemical | ChemComp-SO4 / | #5: Water | ChemComp-HOH / | Sequence details | IN STRAIN PAC1, RESIDUE 47 IS ALA, AS OPPOSED TO THR IN STRAIN PAO1, SEQUENCE DISCREPANCY BETWEEN ...IN STRAIN PAC1, RESIDUE 47 IS ALA, AS OPPOSED TO THR IN STRAIN PAO1, SEQUENCE DISCREPANC | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.35 Å3/Da / Density % sol: 47 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: HANGING DROP VAPOR DIFFUSION. RESERVOIR SOLUTION CONTAINED 100MM SODIUM CACODYLATE AT PH 6.5 OR 100MM TRIS-HCL AT PH7.5, 18-22% (W/V) PEG 10 000 AND 0.1% (W/V) NAN3. PROTEIN CONCENTRATION OF ...Details: HANGING DROP VAPOR DIFFUSION. RESERVOIR SOLUTION CONTAINED 100MM SODIUM CACODYLATE AT PH 6.5 OR 100MM TRIS-HCL AT PH7.5, 18-22% (W/V) PEG 10 000 AND 0.1% (W/V) NAN3. PROTEIN CONCENTRATION OF 10 MG/ML. DROPS KEPT AT 291K. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: May 6, 2003 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 1.44→44.72 Å / Num. obs: 58095 / % possible obs: 93.8 % / Observed criterion σ(I): 0 / Redundancy: 2.8 % / Biso Wilson estimate: 14.2 Å2 / Rmerge(I) obs: 0.04 / Net I/σ(I): 11.6 |
Reflection shell | Resolution: 1.44→1.48 Å / Redundancy: 2.7 % / Rmerge(I) obs: 0.34 / Mean I/σ(I) obs: 3.5 / % possible all: 93.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2BL1 Resolution: 1.44→6 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: DISORDERED RESIDUES WERE MODELED AS ALA
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Solvent computation | Bsol: 22.41 Å2 / ksol: 0.358 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 16.9 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.44→6 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.44→1.49 Å / Num. reflection Rfree: 290 / Num. reflection Rwork: 5347 / Total num. of bins used: 10 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Xplor file |
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