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- PDB-2h9b: Crystal structure of the effector binding domain of a BenM varian... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2h9b | ||||||
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Title | Crystal structure of the effector binding domain of a BenM variant (BenM R156H/T157S) | ||||||
![]() | HTH-type transcriptional regulator benM | ||||||
![]() | TRANSCRIPTION / LTTR / BenM / Transcriptional activator / lysR-type transcriptional regulator | ||||||
Function / homology | ![]() : / protein-DNA complex / DNA-binding transcription factor activity / DNA binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ezezika, O.C. / Craven, S.H. / Neidle, E.L. / Momany, C. | ||||||
![]() | ![]() Title: Inducer responses of BenM, a LysR-type transcriptional regulator from Acinetobacter baylyi ADP1. Authors: Craven, S.H. / Ezezika, O.C. / Haddad, S. / Hall, R.A. / Momany, C. / Neidle, E.L. #1: ![]() Title: Distinct Effector-binding Sites Enable Synergistic Transcriptional Activation By BenM, a LysR-type Regulator Authors: Ezezika, O.C. / Haddad, S. / Clark, T.J. / Neidle, E.L. / Momany, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 122.5 KB | Display | ![]() |
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PDB format | ![]() | 91.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 2h99C ![]() 3glbC ![]() 2f97S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Details | biological unit is a dimer |
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Components
#1: Protein | Mass: 35611.961 Da / Num. of mol.: 2 / Fragment: Effector binding domain / Mutation: R156H, T157S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-SO4 / #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 49.9 % |
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Crystal grow | Temperature: 296.4 K Details: Precipitant:2.0 M ammonium sulfate Protein: 20 mM tris HCl, 0.5 M NaCl, pH 7.9, 10% glycerol Equal volumes mixed, Microbatch under oil, temperature 296.4K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Aug 22, 2005 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→50 Å / Num. obs: 48633 / % possible obs: 100 % / Redundancy: 6.9 % / Rmerge(I) obs: 0.078 / Χ2: 0.858 |
Reflection shell | Resolution: 1.8→1.86 Å / % possible obs: 99.9 % / Redundancy: 6.3 % / Rmerge(I) obs: 0.464 / Num. unique obs: 4763 / Χ2: 0.799 / % possible all: 99.77 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB accession code 2F97, BenM-EBD (high pH) Resolution: 1.8→46.7 Å / Cor.coef. Fo:Fc: 0.957 / Cor.coef. Fo:Fc free: 0.935 / SU B: 4.306 / SU ML: 0.074 / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / ESU R: 0.119 / ESU R Free: 0.117 / Stereochemistry target values: Engh & Huber / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 16.201 Å2
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Refinement step | Cycle: LAST / Resolution: 1.8→46.7 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.8→1.846 Å / Total num. of bins used: 20
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