[English] 日本語
![](img/lk-miru.gif)
- PDB-2gn7: Metal-free (apo) P. angolensis seed lectin in complex with Man-al... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 2gn7 | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Title | Metal-free (apo) P. angolensis seed lectin in complex with Man-alpha(1-3)Man-alpha(1-6)Man | ||||||||||||
![]() | lectin | ||||||||||||
![]() | SUGAR BINDING PROTEIN / legume lectin / metal-free lectin / sugar complex / Man-alpha(1-3)Man-alpha(1-6)Man / beta sandwich | ||||||||||||
Function / homology | ![]() | ||||||||||||
Biological species | ![]() | ||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||
![]() | Garcia-Pino, A. / Buts, L. / Wyns, L. / Loris, R. | ||||||||||||
![]() | ![]() Title: Interplay Between Metal Binding and cis/trans Isomerization in Legume Lectins: Structural and Thermodynamic Study of P. angolensis Lectin. Authors: Garcia-Pino, A. / Buts, L. / Wyns, L. / Loris, R. | ||||||||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 94.7 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 75.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 744.8 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 769.7 KB | Display | |
Data in XML | ![]() | 21.8 KB | Display | |
Data in CIF | ![]() | 28.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2gmeC ![]() 2gmmC ![]() 2gmpC ![]() 2gn3C ![]() 2gnbC ![]() 2gndC ![]() 2gnmC ![]() 2gntC C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
| ||||||||
Details | Subunits A and B together form the lectin dimer |
-
Components
#1: Protein | Mass: 27558.297 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #2: Polysaccharide | beta-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose | Source method: isolated from a genetically manipulated source #3: Sugar | ChemComp-MAN / | #4: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.08 Å3/Da / Density % sol: 40.78 % |
---|---|
Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7.2 Details: 10% PEG6000, 20% MPD, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Feb 24, 2004 |
Radiation | Monochromator: SAGITALLY FOCUSED Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.934 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→15 Å / Num. all: 9174 / Num. obs: 9174 / % possible obs: 85.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 |
Reflection shell | Resolution: 2.9→3 Å / % possible all: 71.3 |
-
Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]()
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.9→15 Å
| ||||||||||||||||||||
Refine LS restraints |
|