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- PDB-2gmm: Metal-free (apo) P. angolensis seed lectin in complex with Man-al... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2gmm | ||||||||||||
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Title | Metal-free (apo) P. angolensis seed lectin in complex with Man-alpha(1-2)Man | ||||||||||||
![]() | lectin | ||||||||||||
![]() | SUGAR BINDING PROTEIN / legume lectin / metal-free lectin / sugar complex / Man2Man / beta sandwich | ||||||||||||
Function / homology | ![]() | ||||||||||||
Biological species | ![]() | ||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||
![]() | Garcia-Pino, A. / Buts, L. / Wyns, L. / Loris, R. | ||||||||||||
![]() | ![]() Title: Interplay Between Metal Binding and cis/trans Isomerization in Legume Lectins: Structural and Thermodynamic Study of P. angolensis Lectin. Authors: Garcia-Pino, A. / Buts, L. / Wyns, L. / Loris, R. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 103.1 KB | Display | ![]() |
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PDB format | ![]() | 81.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 21.6 KB | Display | |
Data in CIF | ![]() | 30.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2gmeC ![]() 2gmpC ![]() 2gn3C ![]() 2gn7C ![]() 2gnbC ![]() 2gndC ![]() 2gnmC ![]() 2gntC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Details | Subunits A and B together form the lectin dimer |
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Components
#1: Protein | Mass: 27558.297 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) ![]() #2: Polysaccharide | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.16 Å3/Da / Density % sol: 43.18 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.2 Details: 10% PEG 6000, 20% MPD, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Jan 1, 2005 |
Radiation | Monochromator: SAGITALLY FOCUSED Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8123 Å / Relative weight: 1 |
Reflection | Resolution: 2.15→25 Å / Num. all: 26425 / Num. obs: 26425 / % possible obs: 98.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 |
Reflection shell | Resolution: 2.15→2.23 Å / % possible all: 96.5 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: metal-free PAL Resolution: 2.15→25 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.15→25 Å
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Refine LS restraints |
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