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- PDB-2e4v: Crystal structure of the extracellular region of the group II met... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2e4v | ||||||
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Title | Crystal structure of the extracellular region of the group II metabotropic glutamate receptor complexed with DCG-IV | ||||||
![]() | Metabotropic glutamate receptor 3 | ||||||
![]() | SIGNALING PROTEIN / G-PROTEIN-COUPLED RECEPTOR / NEURON / CENTRAL NERVE SYSTEM | ||||||
Function / homology | ![]() Class C/3 (Metabotropic glutamate/pheromone receptors) / group II metabotropic glutamate receptor activity / G protein-coupled glutamate receptor signaling pathway / astrocyte projection / G alpha (i) signalling events / postsynaptic modulation of chemical synaptic transmission / calcium channel regulator activity / regulation of synaptic transmission, glutamatergic / sensory perception of pain / modulation of chemical synaptic transmission ...Class C/3 (Metabotropic glutamate/pheromone receptors) / group II metabotropic glutamate receptor activity / G protein-coupled glutamate receptor signaling pathway / astrocyte projection / G alpha (i) signalling events / postsynaptic modulation of chemical synaptic transmission / calcium channel regulator activity / regulation of synaptic transmission, glutamatergic / sensory perception of pain / modulation of chemical synaptic transmission / presynaptic membrane / gene expression / scaffold protein binding / postsynaptic membrane / postsynapse / dendritic spine / postsynaptic density / neuron projection / axon / glutamatergic synapse / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Muto, T. / Tsuchiya, D. / Morikawa, K. / Jingami, H. | ||||||
![]() | ![]() Title: Structures of the extracellular regions of the group II/III metabotropic glutamate receptors Authors: Muto, T. / Tsuchiya, D. / Morikawa, K. / Jingami, H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 225.1 KB | Display | ![]() |
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PDB format | ![]() | 178.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 487.6 KB | Display | ![]() |
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Full document | ![]() | 533.5 KB | Display | |
Data in XML | ![]() | 46.8 KB | Display | |
Data in CIF | ![]() | 64.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2e4uSC ![]() 2e4wC ![]() 2e4xC ![]() 2e4yC ![]() 2e4zC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Details | The asymmetric unit contains one biological homodimer. |
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Components
#1: Protein | Mass: 63117.297 Da / Num. of mol.: 2 / Fragment: Extracellular region / Mutation: N414Q, N439Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Sugar | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.47 Å3/Da / Density % sol: 64.6 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 5 Details: 0.2M NH4H2PO4, 2.5-5.0% PEG 6000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Dec 1, 2004 |
Radiation | Monochromator: SI / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→100 Å / Num. all: 67660 / Num. obs: 67660 / % possible obs: 99.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.073 / Rsym value: 0.064 / Net I/σ(I): 6.7 |
Reflection shell | Resolution: 2.4→2.53 Å / Rmerge(I) obs: 0.553 / Mean I/σ(I) obs: 1.3 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2E4U Resolution: 2.4→20 Å / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.4→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.4→2.49 Å / Rfactor Rfree: 0.3882 / Rfactor Rwork: 0.3683 |