- PDB-2c1t: Structure of the Kap60p:Nup2 complex -
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IDまたはキーワード:
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基本情報
登録情報
データベース: PDB / ID: 2c1t
タイトル
Structure of the Kap60p:Nup2 complex
要素
IMPORTIN ALPHA SUBUNIT
NUCLEOPORIN NUP2
キーワード
PROTEIN TRANSPORT/MEMBRANE PROTEIN / ARMADILLO REPEAT / KARYOPHERIN RECYCLING / NLS RELEASE / NUCEAR IMPORT / NUCLEAR PROTEIN / NUCLEOPORIN / PROTEIN TRANSPORT / NUCLEAR TRANSPORT-COMPLEX / NUCLEAR PORE COMPLEX / PHOSPHORYLATION / TRANSLOCATION / PROTEIN TRANSPORT-MEMBRANE PROTEIN complex
機能・相同性
機能・相同性情報
proteasome localization / mRNA export from nucleus in response to heat stress / protein localization to nuclear inner membrane / transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery / nuclear pore cytoplasmic filaments / import into nucleus / Transport of Mature mRNA derived from an Intron-Containing Transcript / post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery / Regulation of HSF1-mediated heat shock response / nuclear pore nuclear basket ...proteasome localization / mRNA export from nucleus in response to heat stress / protein localization to nuclear inner membrane / transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery / nuclear pore cytoplasmic filaments / import into nucleus / Transport of Mature mRNA derived from an Intron-Containing Transcript / post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery / Regulation of HSF1-mediated heat shock response / nuclear pore nuclear basket / SUMOylation of SUMOylation proteins / importin-alpha family protein binding / NLS-dependent protein nuclear import complex / SUMOylation of RNA binding proteins / structural constituent of nuclear pore / protein targeting to membrane / SUMOylation of chromatin organization proteins / nucleocytoplasmic transport / silent mating-type cassette heterochromatin formation / nuclear import signal receptor activity / poly(A)+ mRNA export from nucleus / nuclear localization sequence binding / NLS-bearing protein import into nucleus / subtelomeric heterochromatin formation / nuclear pore / protein export from nucleus / small GTPase binding / protein import into nucleus / nuclear envelope / disordered domain specific binding / chromosome, telomeric region / nuclear membrane / protein-containing complex binding / perinuclear region of cytoplasm / protein-containing complex / mitochondrion / nucleoplasm / nucleus / cytoplasm / cytosol 類似検索 - 分子機能
IMPORTIN ALPHA SUBUNIT: SPECIFICALLY BINDS TO SUBSTRATES CONTAINING NLS MOTIF AND PROMOTES DOCKING ...IMPORTIN ALPHA SUBUNIT: SPECIFICALLY BINDS TO SUBSTRATES CONTAINING NLS MOTIF AND PROMOTES DOCKING OF IMPORT SUBSTRATES TO THE NUCLEAR ENVELOPE. NUCLEOPORIN NUP2: WORKS AS A COMPONENT OF THE NUCLEAR PORE COMPLEX (NPC). ENGINEERED RESIDUE IN CHAIN A, TYR 397 TO ASP ENGINEERED RESIDUE IN CHAIN B, TYR 397 TO ASP
配列の詳細
RESIDUES 1 TO 87 REMOVED. Y397D INTRODUCED MUTATION ONLY RESIDUES 1 TO 51 PRESENT
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 2.4 Å3/Da / 溶媒含有率: 49 %
結晶化
pH: 6.8 詳細: EXPERIMENTAL DETALS GIVEN IN ENTRY 1UN0 AND IN MATSUURA ET AL, EMBO J, 22, 5358, 2003., pH 6.80
解像度: 2.6→20 Å / Cor.coef. Fo:Fc: 0.943 / Cor.coef. Fo:Fc free: 0.914 / SU B: 10.848 / SU ML: 0.229 / 交差検証法: THROUGHOUT / ESU R: 0.673 / ESU R Free: 0.31 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
Rfactor
反射数
%反射
Selection details
Rfree
0.251
1806
5.1 %
RANDOM
Rwork
0.203
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obs
0.205
33802
97.8 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: BABINET MODEL WITH MASK