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Open data
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Basic information
Entry | Database: PDB / ID: 2bva | ||||||
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Title | Crystal structure of the human P21-activated kinase 4 | ||||||
![]() | P21-ACTIVATED KINASE 4 | ||||||
![]() | TRANSFERASE / PROTEIN KINASE / STE20 / PAK4 / ATP-BINDING | ||||||
Function / homology | ![]() signal transduction => GO:0007165 / dendritic spine development / cadherin binding involved in cell-cell adhesion / stress-activated protein kinase signaling cascade / Activation of RAC1 / RHOV GTPase cycle / activation of protein kinase activity / RHOJ GTPase cycle / RHOQ GTPase cycle / regulation of MAPK cascade ...signal transduction => GO:0007165 / dendritic spine development / cadherin binding involved in cell-cell adhesion / stress-activated protein kinase signaling cascade / Activation of RAC1 / RHOV GTPase cycle / activation of protein kinase activity / RHOJ GTPase cycle / RHOQ GTPase cycle / regulation of MAPK cascade / CDC42 GTPase cycle / RHOH GTPase cycle / RHOU GTPase cycle / cellular response to organic cyclic compound / RHOG GTPase cycle / RAC2 GTPase cycle / RAC3 GTPase cycle / negative regulation of endothelial cell apoptotic process / cytoskeleton organization / RAC1 GTPase cycle / regulation of cell growth / adherens junction / positive regulation of angiogenesis / cell migration / non-specific serine/threonine protein kinase / protein kinase activity / intracellular signal transduction / cell cycle / phosphorylation / protein serine kinase activity / focal adhesion / protein serine/threonine kinase activity / apoptotic process / Golgi apparatus / signal transduction / ATP binding / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Debreczeni, J.E. / Bunkoczi, G. / Eswaran, J. / Filippakopoulos, P. / Das, S. / Fedorov, O. / Sundstrom, M. / Arrowsmith, C. / Edwards, A. / von Delft, F. / Knapp, S. | ||||||
![]() | ![]() Title: Crystal Structures of the P21-Activated Kinases Pak4, Pak5, and Pak6 Reveal Catalytic Domain Plasticity of Active Group II Paks. Authors: Eswaran, J. / Lee, W.H. / Debreczeni, J.E. / Filippakopoulos, P. / Turnbull, A. / Fedorov, O. / Deacon, S.W. / Peterson, J.R. / Knapp, S. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 117.4 KB | Display | ![]() |
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PDB format | ![]() | 90.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 436.6 KB | Display | ![]() |
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Full document | ![]() | 463.1 KB | Display | |
Data in XML | ![]() | 24 KB | Display | |
Data in CIF | ![]() | 31.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2c30C ![]() 2cdzC ![]() 2f57C ![]() 1u5rS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.49972, -0.86616, -0.00676), Vector: |
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Components
#1: Protein | Mass: 33011.371 Da / Num. of mol.: 2 / Fragment: KINASE DOMAIN, RESIDUES 300-591 Source method: isolated from a genetically manipulated source Details: PHOSPHORYLATION ON SER 474 A AND SER 474 B / Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: Q8NCH5, UniProt: O96013*PLUS, EC: 2.7.1.37 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3 Å3/Da / Density % sol: 59 % |
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Crystal grow | Method: vapor diffusion, sitting drop / Details: SITTING DROPS, 1.5 M NACL, 10% ETHANOL |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Apr 23, 2005 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.968 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→40.4 Å / Num. obs: 38417 / % possible obs: 99.8 % / Observed criterion σ(I): 3.5 / Redundancy: 2.7 % / Rmerge(I) obs: 0.06 / Net I/σ(I): 11.86 |
Reflection shell | Resolution: 2.3→2.4 Å / Redundancy: 2.7 % / Rmerge(I) obs: 0.28 / Mean I/σ(I) obs: 3.55 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1U5R Resolution: 2.3→20 Å / Num. parameters: 17176 / Num. restraintsaints: 22479 / Cross valid method: FREE R-VALUE / σ(F): 0 Stereochemistry target values: ENGH AND HUBER BASED ON HIC-UP ENTRY Details: 1. HYDROGENS WERE ADDED ON RIDING POSITIONS 2. TWIN REFINEMENT: PERFECT MEROHEDRAL TWIN, APPARENT SPACEGROUP P3221, REAL SPACEGROUP P32, TWIN LAW: 010 100 00-1, BASF: 0.5006 3. NCS RESTRAINTS WERE USED
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Refine analyze | Num. disordered residues: 0 / Occupancy sum hydrogen: 13065 / Occupancy sum non hydrogen: 12879 | |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.3→20 Å
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Refine LS restraints |
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