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- PDB-2b92: Crystal-structure of the N-terminal Large GTPase Domain of human ... -

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Basic information

Entry
Database: PDB / ID: 2b92
TitleCrystal-structure of the N-terminal Large GTPase Domain of human Guanylate Binding protein 1 (hGBP1) in complex with GDP/AlF3
ComponentsInterferon-induced guanylate-binding protein 1
KeywordsSIGNALING PROTEIN / protein- guanine nucleotide complex
Function / homology
Function and homology information


protein localization to vacuole / negative regulation of substrate adhesion-dependent cell spreading / negative regulation of interleukin-2 production / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / negative regulation of protein localization to plasma membrane / negative regulation of T cell receptor signaling pathway / cytokine binding / spectrin binding / regulation of calcium-mediated signaling / regulation of protein localization to plasma membrane ...protein localization to vacuole / negative regulation of substrate adhesion-dependent cell spreading / negative regulation of interleukin-2 production / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / negative regulation of protein localization to plasma membrane / negative regulation of T cell receptor signaling pathway / cytokine binding / spectrin binding / regulation of calcium-mediated signaling / regulation of protein localization to plasma membrane / vesicle membrane / cellular response to interleukin-1 / cellular response to interferon-gamma / negative regulation of ERK1 and ERK2 cascade / Hsp90 protein binding / GDP binding / Interferon gamma signaling / actin cytoskeleton / cellular response to tumor necrosis factor / actin binding / defense response to virus / cytoplasmic vesicle / GTPase activity / Golgi membrane / GTP binding / Golgi apparatus / enzyme binding / protein homodimerization activity / extracellular region / identical protein binding / plasma membrane / cytosol / cytoplasm
Similarity search - Function
Guanylate-binding protein, C-terminal / Guanylate-binding protein, C-terminal domain / Guanylate-binding protein/Atlastin, C-terminal / Guanylate-binding protein, N-terminal domain / Guanylate-binding protein, C-terminal domain superfamily / Guanylate-binding protein, N-terminal / GB1/RHD3-type guanine nucleotide-binding (G) domain profile. / GB1/RHD3-type guanine nucleotide-binding (G) domain / P-loop containing nucleotide triphosphate hydrolases / P-loop containing nucleoside triphosphate hydrolase ...Guanylate-binding protein, C-terminal / Guanylate-binding protein, C-terminal domain / Guanylate-binding protein/Atlastin, C-terminal / Guanylate-binding protein, N-terminal domain / Guanylate-binding protein, C-terminal domain superfamily / Guanylate-binding protein, N-terminal / GB1/RHD3-type guanine nucleotide-binding (G) domain profile. / GB1/RHD3-type guanine nucleotide-binding (G) domain / P-loop containing nucleotide triphosphate hydrolases / P-loop containing nucleoside triphosphate hydrolase / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
ALUMINUM FLUORIDE / GUANOSINE-5'-DIPHOSPHATE / Guanylate-binding protein 1
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.2 Å
AuthorsGhosh, A. / Praefcke, G.J.K. / Renault, L. / Wittinghofer, A. / Herrmann, C.
Citation
Journal: Nature / Year: 2006
Title: How guanylate-binding proteins achieve assembly-stimulated processive cleavage of GTP to GMP.
Authors: Ghosh, A. / Praefcke, G.J. / Renault, L. / Wittinghofer, A. / Herrmann, C.
#1: Journal: Embo J. / Year: 2000
Title: Triphosphate structure of guanylate-binding protein 1 and implications for nucleotide binding and GTPase mechanism.
Authors: Prakash, B. / Renault, L. / Praefcke, G.J. / Herrmann, C. / Wittinghofer, A.
#2: Journal: Nature / Year: 2000
Title: Structure of human guanylate-binding protein 1 representing a unique class of GTP-binding proteins.
Authors: Prakash, B. / Praefcke, G.J. / Renault, L. / Herrmann, C. / Wittinghofer, A.
History
DepositionOct 10, 2005Deposition site: RCSB / Processing site: RCSB
Revision 1.0Mar 7, 2006Provider: repository / Type: Initial release
Revision 1.1May 1, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Advisory / Version format compliance
Revision 1.3Oct 11, 2017Group: Refinement description / Category: software / Item: _software.classification / _software.name

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Interferon-induced guanylate-binding protein 1
B: Interferon-induced guanylate-binding protein 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)75,2608
Polymers74,1572
Non-polymers1,1036
Water27015
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area6590 Å2
ΔGint-36 kcal/mol
Surface area24410 Å2
MethodPISA
Unit cell
γ
α
β
Length a, b, c (Å)148.820, 103.921, 55.485
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11A
21B
31A
41B
51A
61B
71A
81B
91A
101B
111A
121B

NCS domain segments:

Ens-ID: 1

Dom-IDComponent-IDBeg label comp-IDEnd label comp-IDRefine codeAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11METLYS4AA7 - 6118 - 72
21METLYS4BB7 - 6118 - 72
32LYSSER4AA63 - 15474 - 165
42LYSSER4BB63 - 15474 - 165
53SERLYS4AA173 - 210184 - 221
63SERLYS4BB173 - 210184 - 221
74LYSHIS4AA215 - 243226 - 254
84LYSHIS4BB215 - 243226 - 254
95LEUSER4AA253 - 305264 - 316
105LEUSER4BB253 - 305264 - 316
116GDPMG1AE - C593 - 5951
126GDPMG1BF - D593 - 5951
DetailsThe biological assembly is the homodimer in the asymmetric unit

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Components

#1: Protein Interferon-induced guanylate-binding protein 1 / GTP-binding protein 1 / Guanine nucleotide-binding protein 1 / HuGBP-1


Mass: 37078.449 Da / Num. of mol.: 2 / Fragment: N-terminal Large GTPase domain
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: GBP1 / Plasmid: pQE9 / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: P32455
#2: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Mg
#3: Chemical ChemComp-GDP / GUANOSINE-5'-DIPHOSPHATE / Guanosine diphosphate


Type: RNA linking / Mass: 443.201 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H15N5O11P2 / Comment: GDP, energy-carrying molecule*YM
#4: Chemical ChemComp-AF3 / ALUMINUM FLUORIDE / Aluminium fluoride


Mass: 83.977 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: AlF3
#5: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 15 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.14 Å3/Da / Density % sol: 60.78 %
Crystal growTemperature: 298 K
Details: 12.5% Peg3350, 125mM Na2HPO4 (from Hampton Research peg/ion screen supplied at pH9.1), VAPOR DIFFUSION, HANGING DROP, temperature 298K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: ESRF / Beamline: ID14-4 / Wavelength: 0.9393
DetectorType: ADSC QUANTUM 4 / Detector: CCD / Date: Jun 15, 2002 / Details: GE(220) CRYSTAL
RadiationMonochromator: DIAMOND MONOCHROMATOR / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9393 Å / Relative weight: 1
ReflectionResolution: 3.2→30 Å / Num. obs: 14438 / % possible obs: 97.4 % / Observed criterion σ(I): -3 / Redundancy: 9.8 % / Biso Wilson estimate: 90.1 Å2 / Rsym value: 0.092 / Net I/σ(I): 17.9
Reflection shellResolution: 3.2→3.26 Å / Redundancy: 11.7 % / Mean I/σ(I) obs: 6.8 / Rsym value: 0.457 / % possible all: 95.4

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Processing

Software
NameVersionClassification
REFMAC5.2.0005refinement
MAR345data collection
XDSdata scaling
AMoREphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 2B8W
Resolution: 3.2→30 Å / Cor.coef. Fo:Fc: 0.948 / Cor.coef. Fo:Fc free: 0.925 / SU B: 41.573 / SU ML: 0.323 / TLS residual ADP flag: LIKELY RESIDUAL / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R Free: 0.478 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
RfactorNum. reflection% reflectionSelection details
Rfree0.25 989 6.7 %RANDOM
Rwork0.204 ---
obs0.207 13705 99.3 %-
all-13705 --
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK
Displacement parametersBiso mean: 60.57 Å2
Baniso -1Baniso -2Baniso -3
1--4.33 Å20 Å20 Å2
2--2.36 Å20 Å2
3---1.97 Å2
Refinement stepCycle: LAST / Resolution: 3.2→30 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms4585 0 66 15 4666
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0270.0224752
X-RAY DIFFRACTIONr_bond_other_d
X-RAY DIFFRACTIONr_angle_refined_deg0.6641.9866436
X-RAY DIFFRACTIONr_angle_other_deg
X-RAY DIFFRACTIONr_dihedral_angle_1_deg10.6435574
X-RAY DIFFRACTIONr_dihedral_angle_2_deg40.28624.322199
X-RAY DIFFRACTIONr_dihedral_angle_3_deg22.54115846
X-RAY DIFFRACTIONr_dihedral_angle_4_deg23.6431522
X-RAY DIFFRACTIONr_chiral_restr0.2880.2726
X-RAY DIFFRACTIONr_gen_planes_refined0.0160.023487
X-RAY DIFFRACTIONr_gen_planes_other
X-RAY DIFFRACTIONr_nbd_refined0.2820.21981
X-RAY DIFFRACTIONr_nbd_other
X-RAY DIFFRACTIONr_nbtor_refined0.3410.23187
X-RAY DIFFRACTIONr_nbtor_other
X-RAY DIFFRACTIONr_xyhbond_nbd_refined0.1970.2125
X-RAY DIFFRACTIONr_xyhbond_nbd_other
X-RAY DIFFRACTIONr_metal_ion_refined0.0610.22
X-RAY DIFFRACTIONr_metal_ion_other
X-RAY DIFFRACTIONr_symmetry_vdw_refined0.3210.210
X-RAY DIFFRACTIONr_symmetry_vdw_other
X-RAY DIFFRACTIONr_symmetry_hbond_refined0.020.21
X-RAY DIFFRACTIONr_symmetry_hbond_other
X-RAY DIFFRACTIONr_symmetry_metal_ion_refined
X-RAY DIFFRACTIONr_symmetry_metal_ion_other
X-RAY DIFFRACTIONr_mcbond_it2.4161.52976
X-RAY DIFFRACTIONr_mcbond_other
X-RAY DIFFRACTIONr_mcangle_it3.02624664
X-RAY DIFFRACTIONr_scbond_it4.34932075
X-RAY DIFFRACTIONr_scangle_it5.8674.51772
X-RAY DIFFRACTIONr_rigid_bond_restr
X-RAY DIFFRACTIONr_sphericity_free
X-RAY DIFFRACTIONr_sphericity_bonded
Refine LS restraints NCS

Dom-ID: 1 / Auth asym-ID: A / Ens-ID: 1 / Refine-ID: X-RAY DIFFRACTION

NumberTypeRms dev position (Å)Weight position
33tight positional0.390.05
2120medium positional0.270.5
33tight thermal1.170.5
2120medium thermal0.572
LS refinement shellResolution: 3.2→3.26 Å / Total num. of bins used: 27
RfactorNum. reflection% reflection
Rfree0.38 51 -
Rwork0.271 754 -
obs--95.4 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
11.78680.5675-0.20180.6516-0.04690.45340.09830.0122-0.007-0.00350.02470.0836-0.0272-0.064-0.1230.0790.0432-0.00980.08920.01230.014559.02519.87535.505
21.90330.7693-0.0710.8813-0.10270.62060.11940.03630.05380.0403-0.0286-0.0652-0.0330.07-0.09080.07320.03350.02830.0658-0.00140.01588.576832.12535.0964
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
1X-RAY DIFFRACTION1AA6 - 30817 - 319
2X-RAY DIFFRACTION1AE - C593 - 5951
3X-RAY DIFFRACTION2BB6 - 30817 - 319
4X-RAY DIFFRACTION2BF - D593 - 5951

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