+Open data
-Basic information
Entry | Database: PDB / ID: 1z7t | ||||||
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Title | Solution structure of Bacillus subtilis BLAP apo-form | ||||||
Components | Biotin/Lipoyl Attachment Protein | ||||||
Keywords | TRANSPORT PROTEIN / Bacillus subtilis / single-domain Biotin Carboxyl Carrier Protein / solution structure / apo-form / STRUCTURAL GENOMICS | ||||||
Function / homology | Function and homology information : / RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain / Biotin-requiring enzyme / Biotinyl/lipoyl domain profile. / Biotin/lipoyl attachment / Single hybrid motif / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / Beta Barrel / Mainly Beta Similarity search - Domain/homology | ||||||
Biological species | Bacillus subtilis subsp. subtilis str. 168 (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing, molecular dynamics | ||||||
Authors | Cui, G. / Xia, B. | ||||||
Citation | Journal: To be Published Title: Solution structure of Bacillus subtilis BLAP apo-form Authors: Cui, G. / Xia, B. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1z7t.cif.gz | 436.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1z7t.ent.gz | 366.2 KB | Display | PDB format |
PDBx/mmJSON format | 1z7t.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1z7t_validation.pdf.gz | 343.9 KB | Display | wwPDB validaton report |
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Full document | 1z7t_full_validation.pdf.gz | 414.6 KB | Display | |
Data in XML | 1z7t_validation.xml.gz | 18.8 KB | Display | |
Data in CIF | 1z7t_validation.cif.gz | 35.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/z7/1z7t ftp://data.pdbj.org/pub/pdb/validation_reports/z7/1z7t | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 7920.094 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus subtilis subsp. subtilis str. 168 (bacteria) Species: Bacillus subtilis / Strain: subsp. subtilis str. 168 / Plasmid: pET 21a(+) / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)pLysS / References: UniProt: Q9R9I3, UniProt: C0H419*PLUS |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 3D heteronuclear techniques. |
-Sample preparation
Details | Contents: 1.2mM s-BCCP U-15N, 13C; 50mM phosphate buffer NA; 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 150mM / pH: 7 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing, molecular dynamics / Software ordinal: 1 Details: the structures are based on a total of 4125 restraints. 3731 are NOE-derived distance constraints, 109 dihedral angle restraints,28 distance restraints from hydrogen bonds, 257 charity restraints. | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |