+Open data
-Basic information
Entry | Database: PDB / ID: 2mh8 | ||||||
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Title | GA-79-MBP cs-rosetta structures | ||||||
Components | GA-79-MBP, maltose binding protein | ||||||
Keywords | PROTEIN BINDING / MBP / maltose binding protein / GA / human serum albumin binding protein | ||||||
Function / homology | Albumin-binding domain / GA-like domain / GA-like domain / Immunoglobulin/albumin-binding domain superfamily / : / Helicase, Ruva Protein; domain 3 / Orthogonal Bundle / Mainly Alpha / GA-79-MBP, maltose binding protein Function and homology information | ||||||
Biological species | Streptococcus dysgalactiae (bacteria) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | lowest energy, model1 | ||||||
Authors | He, Y. / Chen, Y. / Porter, L. / Bryan, P. / Orban, J. | ||||||
Citation | Journal: Biophys.J. / Year: 2015 Title: Subdomain interactions foster the design of two protein pairs with 80% sequence identity but different folds. Authors: Porter, L.L. / He, Y. / Chen, Y. / Orban, J. / Bryan, P.N. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2mh8.cif.gz | 175.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2mh8.ent.gz | 152.2 KB | Display | PDB format |
PDBx/mmJSON format | 2mh8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mh/2mh8 ftp://data.pdbj.org/pub/pdb/validation_reports/mh/2mh8 | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 6312.251 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptococcus dysgalactiae (bacteria) / Production host: Escherichia coli (E. coli) / References: UniProt: A0A0J9X1W7*PLUS |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 0.27 mM [U-100% 13C; U-100% 15N] GA-79-MBP, 100 mM potassium phosphate, 95% H2O/5% D2O Solvent system: 95% H2O/5% D2O | ||||||||||||
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Sample |
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Sample conditions | Ionic strength: 0.1 / pH: 7.0 / Pressure: ambient / Temperature: 283 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 3000 / Conformers submitted total number: 10 |