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Yorodumi- PDB-1yf3: T4Dam in Complex with AdoHcy and 13-mer Oligonucleotide Making No... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1yf3 | ||||||
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Title | T4Dam in Complex with AdoHcy and 13-mer Oligonucleotide Making Non- and Semi-specific (~1/4) Contact | ||||||
Components |
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Keywords | Transferase/DNA / T4DAM / METHYLTRANSFERASE / DNA / Transferase-DNA COMPLEX | ||||||
Function / homology | Function and homology information symbiont-mediated evasion of host restriction-modification system / DNA-methyltransferase activity / site-specific DNA-methyltransferase (adenine-specific) / site-specific DNA-methyltransferase (adenine-specific) activity / methylation / DNA replication / DNA binding Similarity search - Function | ||||||
Biological species | Enterobacteria phage T4 (virus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.29 Å | ||||||
Authors | Horton, J.R. / Liebert, K. / Hattman, S. / Jeltsch, A. / Cheng, X. | ||||||
Citation | Journal: Cell(Cambridge,Mass.) / Year: 2005 Title: Transition from Nonspecific to Specific DNA Interactions along the Substrate-Recognition Pathway of Dam Methyltransferase. Authors: Horton, J.R. / Liebert, K. / Hattman, S. / Jeltsch, A. / Cheng, X. #1: Journal: Nat.Struct.Mol.Biol. / Year: 2003 Title: Structure of the bacteriophage T4 DNA adenine methyltransferase Authors: Yang, Z. / Horton, J.R. / Zhou, L. / Zhang, X.J. / Dong, A. / Zhang, X. / Schlagman, S.L. / Kossykh, V. / Cheng, X. | ||||||
History |
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Remark 999 | SEQUENCE 1) Author states that Q139R, Y140F and Q209L reflect conflicts between deposited protein ...SEQUENCE 1) Author states that Q139R, Y140F and Q209L reflect conflicts between deposited protein sequence and translated deposited DNA-->protein sequence. From their electron density, it appears the translated DNA sequence is correct. 2) Residue 119 were modeled (for both A and B) as Tyr in this structure as the electron density does not support an Asp here. The electron denstiy for B119 fits a Tyr residue perfectly. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1yf3.cif.gz | 137.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1yf3.ent.gz | 102.3 KB | Display | PDB format |
PDBx/mmJSON format | 1yf3.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/yf/1yf3 ftp://data.pdbj.org/pub/pdb/validation_reports/yf/1yf3 | HTTPS FTP |
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-Related structure data
Related structure data | 1yfjC 1yflC 1q0sS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-DNA chain , 2 types, 2 molecules CD
#1: DNA chain | Mass: 3935.587 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: Synthesized by New England Biolabs |
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#2: DNA chain | Mass: 4006.621 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: Synthesized by New England Biolabs |
-Protein , 1 types, 2 molecules AB
#3: Protein | Mass: 30509.988 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Enterobacteria phage T4 (virus) / Genus: T4-like viruses / Species: Enterobacteria phage T4 sensu lato / Gene: DAM / Plasmid: pJW2 / Production host: Escherichia coli (E. coli) / Strain (production host): GM 2971 References: UniProt: P04392, site-specific DNA-methyltransferase (adenine-specific) |
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-Non-polymers , 3 types, 208 molecules
#4: Chemical | #5: Chemical | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.41 Å3/Da / Density % sol: 48.9 % | ||||||||||||||||||||||||||||
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 6.4 Details: PEG MME 5000, citrate-phosphate, ammonium sulphate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K | ||||||||||||||||||||||||||||
Components of the solutions |
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-Data collection
Diffraction | Mean temperature: 173 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 17-ID / Wavelength: 1.2826 Å |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Mar 17, 2003 |
Radiation | Monochromator: Si 111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.2826 Å / Relative weight: 1 |
Reflection | Resolution: 2.29→28 Å / Num. obs: 29474 / % possible obs: 95.7 % / Observed criterion σ(F): -3 / Observed criterion σ(I): -3 / Redundancy: 3.8 % / Biso Wilson estimate: 30 Å2 / Rsym value: 0.047 / Net I/σ(I): 22.5 |
Reflection shell | Resolution: 2.29→2.37 Å / Redundancy: 3.8 % / Mean I/σ(I) obs: 9.6 / Rsym value: 0.187 / % possible all: 97.3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1Q0S Resolution: 2.29→28 Å / Isotropic thermal model: anisotropic / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 48.8 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.29→28 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.29→2.37 Å / Rfactor Rfree error: 0.023
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