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- PDB-1uw4: The structural basis of the interaction between nonsense mediated... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1uw4 | ||||||
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Title | The structural basis of the interaction between nonsense mediated decay factors UPF2 and UPF3 | ||||||
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![]() | RNA BINDING PROTEIN / NONSENSE MEDIATED MRNA DECAY PROTEIN / RNA-BINDING PROTEIN / NMD / RNP DOMAIN / MIF4G DOMAIN | ||||||
Function / homology | ![]() positive regulation of mRNA cis splicing, via spliceosome / exon-exon junction complex / mRNA 3'-end processing / Transport of Mature mRNA derived from an Intron-Containing Transcript / RNA Polymerase II Transcription Termination / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / telomeric DNA binding / centriolar satellite / mRNA transport / animal organ regeneration ...positive regulation of mRNA cis splicing, via spliceosome / exon-exon junction complex / mRNA 3'-end processing / Transport of Mature mRNA derived from an Intron-Containing Transcript / RNA Polymerase II Transcription Termination / nuclear-transcribed mRNA catabolic process, nonsense-mediated decay / telomeric DNA binding / centriolar satellite / mRNA transport / animal organ regeneration / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / mRNA export from nucleus / positive regulation of neuron differentiation / mRNA Splicing - Major Pathway / liver development / positive regulation of translation / brain development / Regulation of expression of SLITs and ROBOs / cytoplasmic ribonucleoprotein granule / neuron projection development / mRNA binding / neuronal cell body / nucleolus / perinuclear region of cytoplasm / RNA binding / nucleoplasm / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kadlec, J. / Izaurralde, E. / Cusack, S. | ||||||
![]() | ![]() Title: The Structural Basis for the Interaction between Nonsense-Mediated Mrna Decay Factors Upf2 and Upf3 Authors: Kadlec, J. / Izaurralde, E. / Cusack, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 154.1 KB | Display | ![]() |
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PDB format | ![]() | 123.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | Mass: 10902.323 Da / Num. of mol.: 2 / Fragment: RRM DOMAIN, RESIDUES 50-140 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Protein | Mass: 29278.039 Da / Num. of mol.: 2 / Fragment: MIF4G DOMAIN, RESIDUES 768-1015 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.9 Å3/Da / Density % sol: 57.2 % | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 6.5 Details: 100 MM AMMONIUM ACETATE, 15 MM MAGNESIUM ACETATE 50 MM SODIUM CACODYLATE 6.5, 8% ISOPROPANOL, pH 6.50 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / pH: 7 / Method: vapor diffusion | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||
Detector | Type: ADSC CCD / Detector: CCD / Date: Apr 16, 2003 / Details: TOROIDAL MIRROR | ||||||||||||
Radiation | Monochromator: SI(111) / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Resolution: 1.95→33.7 Å / Num. obs: 79316 / % possible obs: 99.5 % / Redundancy: 4.3 % / Rmerge(I) obs: 0.064 / Net I/σ(I): 13.74 | ||||||||||||
Reflection shell | Resolution: 1.95→2.1 Å / Redundancy: 4 % / Rmerge(I) obs: 0.3 / Mean I/σ(I) obs: 4.3 / % possible all: 98.6 | ||||||||||||
Reflection | *PLUS Highest resolution: 1.95 Å / Lowest resolution: 30 Å / Redundancy: 4.3 % / Num. measured all: 338871 / Rmerge(I) obs: 0.064 | ||||||||||||
Reflection shell | *PLUS % possible obs: 98.6 % / Rmerge(I) obs: 0.305 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 25.68 Å2
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Refinement step | Cycle: LAST / Resolution: 1.95→30 Å
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Refine LS restraints |
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