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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1tco | ||||||
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タイトル | TERNARY COMPLEX OF A CALCINEURIN A FRAGMENT, CALCINEURIN B, FKBP12 AND THE IMMUNOSUPPRESSANT DRUG FK506 (TACROLIMUS) | ||||||
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![]() | COMPLEX (HYDROLASE/ISOMERASE) / COMPLEX (HYDROLASE-ISOMERASE) / IMMUNOSUPPRESSANT / COMPLEX (HYDROLASE-ISOMERASE) complex | ||||||
機能・相同性 | ![]() mTORC1-mediated signalling / TGF-beta receptor signaling activates SMADs / CLEC7A (Dectin-1) induces NFAT activation / regulation of activin receptor signaling pathway / Calcineurin activates NFAT / FCERI mediated Ca+2 mobilization / Activation of BAD and translocation to mitochondria / calmodulin-dependent protein phosphatase activity / calcineurin complex / calcineurin-mediated signaling ...mTORC1-mediated signalling / TGF-beta receptor signaling activates SMADs / CLEC7A (Dectin-1) induces NFAT activation / regulation of activin receptor signaling pathway / Calcineurin activates NFAT / FCERI mediated Ca+2 mobilization / Activation of BAD and translocation to mitochondria / calmodulin-dependent protein phosphatase activity / calcineurin complex / calcineurin-mediated signaling / macrolide binding / activin receptor binding / lung epithelial cell differentiation / cytoplasmic side of membrane / calcineurin-NFAT signaling cascade / signaling receptor inhibitor activity / transforming growth factor beta receptor binding / TGFBR1 LBD Mutants in Cancer / activin binding / myelination in peripheral nervous system / type I transforming growth factor beta receptor binding / negative regulation of activin receptor signaling pathway / heart trabecula formation / terminal cisterna / ryanodine receptor complex / I-SMAD binding / negative regulation of release of sequestered calcium ion into cytosol / regulation of amyloid precursor protein catabolic process / response to caffeine / Ca2+ pathway / protein maturation by protein folding / ventricular cardiac muscle tissue morphogenesis / 'de novo' protein folding / branching involved in blood vessel morphogenesis / negative regulation of phosphoprotein phosphatase activity / postsynaptic modulation of chemical synaptic transmission / FK506 binding / protein-serine/threonine phosphatase / SMAD binding / mTORC1-mediated signalling / TGF-beta receptor signaling activates SMADs / phosphoprotein phosphatase activity / Calcineurin activates NFAT / regulation of immune response / negative regulation of ryanodine-sensitive calcium-release channel activity / epithelial to mesenchymal transition / phosphatase binding / calcium channel inhibitor activity / protein peptidyl-prolyl isomerization / T cell proliferation / heart morphogenesis / supramolecular fiber organization / dephosphorylation / release of sequestered calcium ion into cytosol / regulation of ryanodine-sensitive calcium-release channel activity / sarcoplasmic reticulum membrane / T cell activation / protein dephosphorylation / negative regulation of protein phosphorylation / TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) / peptidylprolyl isomerase / positive regulation of protein ubiquitination / sarcoplasmic reticulum / peptidyl-prolyl cis-trans isomerase activity / muscle contraction / calcium ion transmembrane transport / negative regulation of transforming growth factor beta receptor signaling pathway / Schaffer collateral - CA1 synapse / sarcolemma / cytokine-mediated signaling pathway / Z disc / response to calcium ion / protein import into nucleus / SARS-CoV-1 activates/modulates innate immune responses / regulation of protein localization / protein folding / positive regulation of protein binding / heart development / protein refolding / postsynapse / positive regulation of canonical NF-kappaB signal transduction / transmembrane transporter binding / amyloid fibril formation / Potential therapeutics for SARS / dendritic spine / calmodulin binding / calcium ion binding / protein homodimerization activity / extracellular exosome / membrane / metal ion binding / cytoplasm / cytosol 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() | ||||||
手法 | ![]() | ||||||
![]() | Griffith, J.P. / Kim, J.L. / Kim, E.E. / Sintchak, M.D. / Thomson, J.A. / Fitzgibbon, M.J. / Fleming, M.A. / Caron, P.R. / Hsiao, K. / Navia, M.A. | ||||||
![]() | ![]() タイトル: X-ray structure of calcineurin inhibited by the immunophilin-immunosuppressant FKBP12-FK506 complex. 著者: Griffith, J.P. / Kim, J.L. / Kim, E.E. / Sintchak, M.D. / Thomson, J.A. / Fitzgibbon, M.J. / Fleming, M.A. / Caron, P.R. / Hsiao, K. / Navia, M.A. | ||||||
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 162.9 KB | 表示 | ![]() |
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PDB形式 | ![]() | 123.3 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 521.6 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 541 KB | 表示 | |
XML形式データ | ![]() | 16.6 KB | 表示 | |
CIF形式データ | ![]() | 28.7 KB | 表示 | |
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-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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要素
-SERINE/THREONINE PHOSPHATASE ... , 2種, 2分子 AB
#1: タンパク質 | 分子量: 43012.969 Da / 分子数: 1 断片: CHAIN A IS THE CATALYTIC SUBUNIT, RESIDUES 18 -392. CHAIN B IS THE REGULATORY SUBUNIT, RESIDUES 1 - 169 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() 参照: UniProt: P48452, protein-serine/threonine phosphatase |
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#2: タンパク質 | 分子量: 19191.709 Da / 分子数: 1 断片: CHAIN A IS THE CATALYTIC SUBUNIT, RESIDUES 18 -392. CHAIN B IS THE REGULATORY SUBUNIT, RESIDUES 1 - 169 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() 参照: UniProt: P63099, protein-serine/threonine phosphatase |
-タンパク質 , 1種, 1分子 C
#3: タンパク質 | 分子量: 11750.392 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() 参照: UniProt: P62942, UniProt: P18203*PLUS, peptidylprolyl isomerase |
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-非ポリマー , 7種, 719分子 ![](data/chem/img/ZN.gif)
![](data/chem/img/FE.gif)
![](data/chem/img/PO4.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/MYR.gif)
![](data/chem/img/FK5.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/FE.gif)
![](data/chem/img/PO4.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/MYR.gif)
![](data/chem/img/FK5.gif)
![](data/chem/img/HOH.gif)
#4: 化合物 | ChemComp-ZN / | ||||||
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#5: 化合物 | ChemComp-FE / | ||||||
#6: 化合物 | ChemComp-PO4 / | ||||||
#7: 化合物 | ChemComp-CA / #8: 化合物 | ChemComp-MYR / | #9: 化合物 | ChemComp-FK5 / | #10: 水 | ChemComp-HOH / | |
-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 3.35 Å3/Da / 溶媒含有率: 63.34 % | ||||||||||||||||||||
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結晶化 | *PLUS 温度: 4 ℃ / 手法: 蒸気拡散法, ハンギングドロップ法 | ||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
放射光源 | 波長: 1.5418 |
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検出器 | タイプ: RIGAKU RAXIS / 検出器: IMAGE PLATE / 日付: 1995 |
放射 | 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.5418 Å / 相対比: 1 |
反射 | Num. obs: 41097 / % possible obs: 91.3 % / Observed criterion σ(I): 0 / 冗長度: 3 % |
反射 | *PLUS 最高解像度: 2.3 Å / Rmerge(I) obs: 0.05 |
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解析
ソフトウェア |
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精密化 | 解像度: 2.5→7 Å / σ(F): 1.5 /
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精密化ステップ | サイクル: LAST / 解像度: 2.5→7 Å
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拘束条件 |
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