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- PDB-1tco: TERNARY COMPLEX OF A CALCINEURIN A FRAGMENT, CALCINEURIN B, FKBP1... -
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Basic information
Entry | Database: PDB / ID: 1tco | ||||||
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Title | TERNARY COMPLEX OF A CALCINEURIN A FRAGMENT, CALCINEURIN B, FKBP12 AND THE IMMUNOSUPPRESSANT DRUG FK506 (TACROLIMUS) | ||||||
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![]() | COMPLEX (HYDROLASE/ISOMERASE) / COMPLEX (HYDROLASE-ISOMERASE) / IMMUNOSUPPRESSANT / COMPLEX (HYDROLASE-ISOMERASE) complex | ||||||
Function / homology | ![]() mTORC1-mediated signalling / TGF-beta receptor signaling activates SMADs / CLEC7A (Dectin-1) induces NFAT activation / regulation of activin receptor signaling pathway / Calcineurin activates NFAT / FCERI mediated Ca+2 mobilization / Activation of BAD and translocation to mitochondria / calmodulin-dependent protein phosphatase activity / calcineurin complex / calcineurin-mediated signaling ...mTORC1-mediated signalling / TGF-beta receptor signaling activates SMADs / CLEC7A (Dectin-1) induces NFAT activation / regulation of activin receptor signaling pathway / Calcineurin activates NFAT / FCERI mediated Ca+2 mobilization / Activation of BAD and translocation to mitochondria / calmodulin-dependent protein phosphatase activity / calcineurin complex / calcineurin-mediated signaling / macrolide binding / activin receptor binding / lung epithelial cell differentiation / cytoplasmic side of membrane / calcineurin-NFAT signaling cascade / signaling receptor inhibitor activity / transforming growth factor beta receptor binding / TGFBR1 LBD Mutants in Cancer / myelination in peripheral nervous system / activin binding / type I transforming growth factor beta receptor binding / negative regulation of activin receptor signaling pathway / heart trabecula formation / terminal cisterna / ryanodine receptor complex / I-SMAD binding / negative regulation of release of sequestered calcium ion into cytosol / regulation of amyloid precursor protein catabolic process / Ca2+ pathway / response to caffeine / protein maturation by protein folding / ventricular cardiac muscle tissue morphogenesis / 'de novo' protein folding / branching involved in blood vessel morphogenesis / negative regulation of phosphoprotein phosphatase activity / postsynaptic modulation of chemical synaptic transmission / FK506 binding / protein-serine/threonine phosphatase / mTORC1-mediated signalling / SMAD binding / TGF-beta receptor signaling activates SMADs / phosphoprotein phosphatase activity / Calcineurin activates NFAT / regulation of immune response / negative regulation of ryanodine-sensitive calcium-release channel activity / epithelial to mesenchymal transition / calcium channel inhibitor activity / phosphatase binding / protein peptidyl-prolyl isomerization / T cell proliferation / supramolecular fiber organization / heart morphogenesis / dephosphorylation / release of sequestered calcium ion into cytosol / regulation of ryanodine-sensitive calcium-release channel activity / sarcoplasmic reticulum membrane / T cell activation / protein dephosphorylation / TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition) / negative regulation of protein phosphorylation / peptidylprolyl isomerase / positive regulation of protein ubiquitination / sarcoplasmic reticulum / peptidyl-prolyl cis-trans isomerase activity / muscle contraction / calcium ion transmembrane transport / negative regulation of transforming growth factor beta receptor signaling pathway / Schaffer collateral - CA1 synapse / sarcolemma / cytokine-mediated signaling pathway / Z disc / response to calcium ion / protein import into nucleus / SARS-CoV-1 activates/modulates innate immune responses / regulation of protein localization / protein folding / positive regulation of protein binding / heart development / protein refolding / postsynapse / positive regulation of canonical NF-kappaB signal transduction / transmembrane transporter binding / amyloid fibril formation / Potential therapeutics for SARS / dendritic spine / calmodulin binding / calcium ion binding / protein homodimerization activity / extracellular exosome / membrane / metal ion binding / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Griffith, J.P. / Kim, J.L. / Kim, E.E. / Sintchak, M.D. / Thomson, J.A. / Fitzgibbon, M.J. / Fleming, M.A. / Caron, P.R. / Hsiao, K. / Navia, M.A. | ||||||
![]() | ![]() Title: X-ray structure of calcineurin inhibited by the immunophilin-immunosuppressant FKBP12-FK506 complex. Authors: Griffith, J.P. / Kim, J.L. / Kim, E.E. / Sintchak, M.D. / Thomson, J.A. / Fitzgibbon, M.J. / Fleming, M.A. / Caron, P.R. / Hsiao, K. / Navia, M.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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PDBx/mmCIF format | ![]() | 162.9 KB | Display | ![]() |
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PDB format | ![]() | 123.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 521.6 KB | Display | ![]() |
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Full document | ![]() | 541 KB | Display | |
Data in XML | ![]() | 16.6 KB | Display | |
Data in CIF | ![]() | 28.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-SERINE/THREONINE PHOSPHATASE ... , 2 types, 2 molecules AB
#1: Protein | Mass: 43012.969 Da / Num. of mol.: 1 Fragment: CHAIN A IS THE CATALYTIC SUBUNIT, RESIDUES 18 -392. CHAIN B IS THE REGULATORY SUBUNIT, RESIDUES 1 - 169 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P48452, protein-serine/threonine phosphatase |
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#2: Protein | Mass: 19191.709 Da / Num. of mol.: 1 Fragment: CHAIN A IS THE CATALYTIC SUBUNIT, RESIDUES 18 -392. CHAIN B IS THE REGULATORY SUBUNIT, RESIDUES 1 - 169 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P63099, protein-serine/threonine phosphatase |
-Protein , 1 types, 1 molecules C
#3: Protein | Mass: 11750.392 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P62942, UniProt: P18203*PLUS, peptidylprolyl isomerase |
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-Non-polymers , 7 types, 719 molecules ![](data/chem/img/ZN.gif)
![](data/chem/img/FE.gif)
![](data/chem/img/PO4.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/MYR.gif)
![](data/chem/img/FK5.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/FE.gif)
![](data/chem/img/PO4.gif)
![](data/chem/img/CA.gif)
![](data/chem/img/MYR.gif)
![](data/chem/img/FK5.gif)
![](data/chem/img/HOH.gif)
#4: Chemical | ChemComp-ZN / | ||||||
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#5: Chemical | ChemComp-FE / | ||||||
#6: Chemical | ChemComp-PO4 / | ||||||
#7: Chemical | ChemComp-CA / #8: Chemical | ChemComp-MYR / | #9: Chemical | ChemComp-FK5 / | #10: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.35 Å3/Da / Density % sol: 63.34 % | ||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 4 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: RIGAKU RAXIS / Detector: IMAGE PLATE / Date: 1995 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 41097 / % possible obs: 91.3 % / Observed criterion σ(I): 0 / Redundancy: 3 % |
Reflection | *PLUS Highest resolution: 2.3 Å / Rmerge(I) obs: 0.05 |
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Processing
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Refinement | Resolution: 2.5→7 Å / σ(F): 1.5
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Refinement step | Cycle: LAST / Resolution: 2.5→7 Å
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