+Open data
-Basic information
Entry | Database: PDB / ID: 1sfe | ||||||
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Title | ADA O6-METHYLGUANINE-DNA METHYLTRANSFERASE FROM ESCHERICHIA COLI | ||||||
Components | ADA O6-METHYLGUANINE-DNA METHYLTRANSFERASE | ||||||
Keywords | DNA BINDING PROTEIN / ENZYME / TRANSFERASE / METHYLTRANSFERASE / NUCLEIC ACID BINDING PROTEIN / DNA REPAIR PROTEIN / DNA-BINDING PROTEIN | ||||||
Function / homology | Function and homology information : / methylated-DNA-[protein]-cysteine S-methyltransferase / methylated-DNA-[protein]-cysteine S-methyltransferase activity / : / DNA alkylation repair / sequence-specific DNA binding / methylation / DNA-binding transcription factor activity / negative regulation of DNA-templated transcription / DNA damage response ...: / methylated-DNA-[protein]-cysteine S-methyltransferase / methylated-DNA-[protein]-cysteine S-methyltransferase activity / : / DNA alkylation repair / sequence-specific DNA binding / methylation / DNA-binding transcription factor activity / negative regulation of DNA-templated transcription / DNA damage response / positive regulation of DNA-templated transcription / zinc ion binding Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.1 Å | ||||||
Authors | Moore, M.H. / Gulbis, J.M. / Dodson, E.J. / Demple, B. / Moody, P.C.E. | ||||||
Citation | Journal: EMBO J. / Year: 1994 Title: Crystal structure of a suicidal DNA repair protein: the Ada O6-methylguanine-DNA methyltransferase from E. coli. Authors: Moore, M.H. / Gulbis, J.M. / Dodson, E.J. / Demple, B. / Moody, P.C. #1: Journal: Novel Approaches in Anticancer Drug Design : Molecular Modelling--New Treatment Strategies (in: Contrib.Oncol., V.49) Year: 1995 Title: Crystal Structure of E.Coli O6-Methylguanine-DNA Methyltransferase Authors: Moody, P.C.E. / Moore, M.H. #2: Journal: J.Mol.Biol. / Year: 1988 Title: Crystallization of O6-Methylguanine-DNA Methyltransferase from Escherichia Coli Authors: Moody, P.C. / Demple, B. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1sfe.cif.gz | 47.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1sfe.ent.gz | 32.7 KB | Display | PDB format |
PDBx/mmJSON format | 1sfe.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1sfe_validation.pdf.gz | 418.5 KB | Display | wwPDB validaton report |
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Full document | 1sfe_full_validation.pdf.gz | 421.6 KB | Display | |
Data in XML | 1sfe_validation.xml.gz | 10.3 KB | Display | |
Data in CIF | 1sfe_validation.cif.gz | 14.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sf/1sfe ftp://data.pdbj.org/pub/pdb/validation_reports/sf/1sfe | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 19766.586 Da / Num. of mol.: 1 / Fragment: C-TERMINAL 19KD OF THE ADA PROTEIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: B / Description: TAC CONTROL / Gene: ADAC / Variant: JM 101 / Plasmid: PADAC / Gene (production host): ADAC / Production host: Escherichia coli (E. coli) References: UniProt: P06134, methylated-DNA-[protein]-cysteine S-methyltransferase |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.27 Å3/Da / Density % sol: 48 % | ||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: microdialysis / PH range low: 8 / PH range high: 7.8 | ||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction |
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Diffraction source |
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Detector |
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Radiation |
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Radiation wavelength |
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Reflection | Num. obs: 9017 / % possible obs: 86 % / Observed criterion σ(I): 0 / Redundancy: 2.2 % / Rmerge(I) obs: 0.058 | ||||||||||||||||||
Reflection | *PLUS Highest resolution: 2.1 Å / Lowest resolution: 40 Å / Num. measured all: 19437 |
-Processing
Software |
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Refinement | Resolution: 2.1→39 Å / σ(F): 0 /
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Displacement parameters | Biso mean: 44.9 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati sigma a obs: 0.15 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→39 Å
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Refine LS restraints |
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Software | *PLUS Name: PROLSQ / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.219 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |