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Yorodumi- PDB-1ryz: Uridine Phosphorylase from Salmonella typhimurium. Crystal Struct... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1ryz | ||||||
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Title | Uridine Phosphorylase from Salmonella typhimurium. Crystal Structure at 2.9 A Resolution | ||||||
Components | Uridine phosphorylase | ||||||
Keywords | TRANSFERASE / uridine phosphorylase / nucleoside phosphorylase | ||||||
Function / homology | Function and homology information uridine phosphorylase / nucleotide catabolic process / uridine phosphorylase activity / UMP salvage / nucleoside catabolic process / cytosol Similarity search - Function | ||||||
Biological species | Salmonella typhimurium (bacteria) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.9 Å | ||||||
Authors | Dontsova, M.V. / Gabdoulkhakov, A.G. / Lyashenko, A.V. / Nikonov, S.V. / Ealick, S.E. / Mikhailov, A.M. | ||||||
Citation | Journal: TO BE PUBLISHED Title: Structure-functions studies of uridine phosphorylase from Salmonella typhimurium Authors: Dontsova, M.V. / Gabdoulkhakov, A.G. / Lyashenko, A.V. / Nikonov, S.V. / Ealick, S.E. / Mikhailov, A.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ryz.cif.gz | 276.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ryz.ent.gz | 223.4 KB | Display | PDB format |
PDBx/mmJSON format | 1ryz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ry/1ryz ftp://data.pdbj.org/pub/pdb/validation_reports/ry/1ryz | HTTPS FTP |
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-Related structure data
Related structure data | 1k3fS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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3 |
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4 |
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Unit cell |
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-Components
#1: Protein | Mass: 27169.092 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Salmonella typhimurium (bacteria) / Strain: LT2 / Gene: udp / Plasmid: pBluescript IISK / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P0A1F6, uridine phosphorylase #2: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.02 Å3/Da / Density % sol: 38.98 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, hanging drop / pH: 4.6 Details: 0.1M Na Acetate trihydrate, 10% PEG 8000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: ROTATING ANODE / Type: OTHER / Wavelength: 1.54179 Å |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Aug 15, 2003 / Details: Capillary optics |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54179 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→30 Å / Num. all: 75053 / Num. obs: 26462 / % possible obs: 93 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 2.84 % / Biso Wilson estimate: -0.2 Å2 / Rmerge(I) obs: 0.154 / Rsym value: 0.177 / Net I/σ(I): 6.89 |
Reflection shell | Resolution: 2.9→2.95 Å / Redundancy: 2.79 % / Rmerge(I) obs: 0.338 / Mean I/σ(I) obs: 3.05 / Num. unique all: 1074 / Rsym value: 0.333 / % possible all: 75.9 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entry 1K3F Resolution: 2.9→29.46 Å / Rfactor Rfree error: 0.008 / Data cutoff high absF: 3363606.7 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 2 / Stereochemistry target values: Engh & Huber / Details: BULK SOLVENT MODEL USED
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 44.0393 Å2 / ksol: 0.301264 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 18.5 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.9→29.46 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.9→3.08 Å / Rfactor Rfree error: 0.02 / Total num. of bins used: 6
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Xplor file |
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