sequence In this streptavidin mutant (M2) the L3,4 loop (sequence: GNAES) was substituted with the ...sequence In this streptavidin mutant (M2) the L3,4 loop (sequence: GNAES) was substituted with the loop from avidin (sequence: TATSNEIK)
構造決定の手法: 分子置換 / 解像度: 1.14→56.8 Å / Cor.coef. Fo:Fc: 0.963 / Cor.coef. Fo:Fc free: 0.954 / SU B: 0.508 / SU ML: 0.025 / 交差検証法: THROUGHOUT / ESU R: 0.041 / ESU R Free: 0.04 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.20029
7818
5 %
RANDOM
Rwork
0.17914
-
-
-
all
0.182
-
-
-
obs
0.18022
148143
96.97 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: BABINET MODEL WITH MASK