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- PDB-1p2f: Crystal Structure Analysis of Response Regulator DrrB, a Thermoto... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1p2f | ||||||
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Title | Crystal Structure Analysis of Response Regulator DrrB, a Thermotoga maritima OmpR/PhoB Homolog | ||||||
![]() | Response Regulator | ||||||
![]() | TRANSCRIPTION / response regulator / DrrB / OmpR/PhoB | ||||||
Function / homology | ![]() phosphorelay response regulator activity / protein-DNA complex / transcription cis-regulatory region binding / regulation of DNA-templated transcription / metal ion binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Robinson, V.L. / Wu, T. / Stock, A.M. | ||||||
![]() | ![]() Title: Structural analysis of the domain interface in DrrB, a response regulator of the OmpR/PhoB subfamily Authors: Robinson, V.L. / Wu, T. / Stock, A.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 55 KB | Display | ![]() |
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PDB format | ![]() | 43.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 426 KB | Display | ![]() |
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Full document | ![]() | 430.2 KB | Display | |
Data in XML | ![]() | 11.9 KB | Display | |
Data in CIF | ![]() | 16.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Details | The biological assembly is a monomer |
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Components
#1: Protein | Mass: 25793.184 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.45 Å3/Da / Density % sol: 49.4 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 310 K / Method: vapor diffusion, hanging drop / pH: 6.3 Details: sodium and potassium phosphate, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 310K | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 27 ℃ / pH: 8 | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Nov 11, 2002 |
Radiation | Monochromator: KOHZU double crystal monochromator with a sagittally focused second crystal Protocol: SAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9786 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→30 Å / Num. all: 44556 / Num. obs: 44556 / % possible obs: 97.4 % / Observed criterion σ(I): 2 |
Reflection shell | Resolution: 1.8→1.86 Å / % possible all: 87.6 |
Reflection | *PLUS Highest resolution: 1.8 Å / Lowest resolution: 25 Å / Redundancy: 7.2 % / Num. measured all: 424245 / Rmerge(I) obs: 0.066 |
Reflection shell | *PLUS Highest resolution: 1.8 Å / % possible obs: 87.6 % / Rmerge(I) obs: 0.32 / Mean I/σ(I) obs: 2.35 |
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Processing
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Refinement | Method to determine structure: ![]()
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.8→20 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 1.8 Å / Num. reflection obs: 40239 / Num. reflection Rfree: 4324 / % reflection Rfree: 10 % | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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