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Open data
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Basic information
Entry | Database: PDB / ID: 1orc | ||||||
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Title | CRO REPRESSOR INSERTION MUTANT K56-[DGEVK] | ||||||
![]() | CRO REPRESSOR INSERTION MUTANT K56-[DGEVK] | ||||||
![]() | GENE REGULATING PROTEIN | ||||||
Function / homology | ![]() latency-replication decision / release from viral latency / negative regulation of transcription by competitive promoter binding / negative regulation of viral transcription / core promoter sequence-specific DNA binding / response to UV / protein homodimerization activity / DNA binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Albright, R.A. / Mossing, M.C. / Matthews, B.W. | ||||||
![]() | ![]() Title: High-resolution structure of an engineered Cro monomer shows changes in conformation relative to the native dimer. Authors: Albright, R.A. / Mossing, M.C. / Matthews, B.W. #1: ![]() Title: Stable, Monomeric Variants of Lambda Cro Obtained by Insertion of a Designed Beta-Hairpin Sequence Authors: Mossing, M.C. / Sauer, R.T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 25.2 KB | Display | ![]() |
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PDB format | ![]() | 15.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 7905.048 Da / Num. of mol.: 1 / Mutation: INS(K56-DGEVK) Source method: isolated from a genetically manipulated source Details: RESULTS IN A 71-RESIDUE STABLE "MONOMER" MUTANT / Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.08 Å3/Da / Density % sol: 40.87 % | ||||||||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 7 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: XUONG-HAMLIN MULTIWIRE / Detector: AREA DETECTOR / Date: Sep 26, 1993 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 9834 / % possible obs: 95.8 % / Observed criterion σ(I): 0 / Redundancy: 4.6 % / Rmerge(I) obs: 0.029 |
Reflection | *PLUS Highest resolution: 1.54 Å / Num. measured all: 45435 |
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Processing
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Refinement | Resolution: 1.54→20 Å / σ(F): 0 Details: BOTH TERMINI OF CRO K56-[DGEVK]ARE DISORDERED. RESIDUES 1, 2, 62, 63, 64, 65, AND 66 HAVE NO INTERPRETABLE DENSITY AND ARE NOT INCLUDED IN THE MODEL. RESIDUES 3 AND 61 EXTEN AWAY FROM THE ...Details: BOTH TERMINI OF CRO K56-[DGEVK]ARE DISORDERED. RESIDUES 1, 2, 62, 63, 64, 65, AND 66 HAVE NO INTERPRETABLE DENSITY AND ARE NOT INCLUDED IN THE MODEL. RESIDUES 3 AND 61 EXTEN AWAY FROM THE GLOBULAR PORTION OF THE MOLECULE AND ARE MOST PROBABLY IN MULTIPLE CONFORMATIONS. THE GLOBULAR PORTION OF THE MOLECULE (RESIDUES 4 - 59) IS EXCEPTIONALLY WELL-DEFINED IN THE DENSITY.
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Refinement step | Cycle: LAST / Resolution: 1.54→20 Å
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Refine LS restraints |
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Software | *PLUS Name: TNT / Classification: refinement | ||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.178 | ||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |