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Yorodumi- PDB-1ofh: Asymmetric complex between HslV and I-domain deleted HslU (H. inf... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1ofh | ||||||
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Title | Asymmetric complex between HslV and I-domain deleted HslU (H. influenzae) | ||||||
Components | (ATP-DEPENDENT ...) x 2 | ||||||
Keywords | HYDROLASE / CHAPERONE / ATP-BINDING | ||||||
Function / homology | Function and homology information HslU-HslV peptidase / HslUV protease complex / proteasome-activating activity / proteasome core complex / protein unfolding / threonine-type endopeptidase activity / proteolysis involved in protein catabolic process / peptidase activity / ATP hydrolysis activity / ATP binding / metal ion binding Similarity search - Function | ||||||
Biological species | HAEMOPHILUS INFLUENZAE (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.5 Å | ||||||
Authors | Kwon, A.R. / Kessler, B.M. / Overkleeft, H.S. / McKay, D.B. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2003 Title: Structure and Reactivity of an Asymmetric Complex between Hslv and I-Domain Deleted Hslu, a Prokaryotic Homolog of the Eukaryotic Proteasome Authors: Kwon, A.R. / Kessler, B.M. / Overkleeft, H.S. / Mckay, D.B. #1: Journal: J.Mol.Biol. / Year: 2002 Title: Structure of Hsluv Complexed with a Vinyl Sulfone Inhibitor: Corroboration of a Proposed Mechanism of Allosteric Activation of Hslv by Hslu Authors: Sousa, M.C. / Kessler, B.M. / Overkleeft, H.S. / Mckay, D.B. #2: Journal: Cell(Cambridge,Mass.) / Year: 2000 Title: Crystal and Solution Structures of an Hsluv Protease-Chaperone Complex Authors: Sousa, M.C. / Trame, C.B. / Tsuruta, H. / Wilbanks, S.M. / Reddy, V.S. / Mckay, D.B. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ofh.cif.gz | 382.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ofh.ent.gz | 311.5 KB | Display | PDB format |
PDBx/mmJSON format | 1ofh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/of/1ofh ftp://data.pdbj.org/pub/pdb/validation_reports/of/1ofh | HTTPS FTP |
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-Related structure data
Related structure data | 1ofiC 1g3iS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
-ATP-DEPENDENT ... , 2 types, 9 molecules ABCGHILMN
#1: Protein | Mass: 34126.020 Da / Num. of mol.: 3 / Fragment: RESIDUES 1-107,244-444 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HAEMOPHILUS INFLUENZAE (bacteria) / Strain: RD / Plasmid: PET / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P43773 #2: Protein | Mass: 18903.549 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HAEMOPHILUS INFLUENZAE (bacteria) / Strain: RD / Plasmid: PET / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) References: UniProt: P43772, Hydrolases; Acting on peptide bonds (peptidases); Threonine endopeptidases |
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-Non-polymers , 4 types, 98 molecules
#3: Chemical | #4: Chemical | ChemComp-MG / #5: Chemical | #6: Water | ChemComp-HOH / | |
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-Details
Compound details | FUNCTION: CHAPERONE SUBUNIT OF A PROTEASOME |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.86 Å3/Da / Density % sol: 57 % | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 5.5 / Details: PEG-MME 2000, KCL,MG(OAC)2,CITRATE, PH 5.5 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7 / Method: unknown | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL11-1 / Wavelength: 0.98 |
Detector | Type: ADSC CCD / Detector: CCD / Date: Mar 15, 2002 / Details: MIRRORS |
Radiation | Monochromator: SI (111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→40 Å / Num. obs: 81671 / % possible obs: 97 % / Observed criterion σ(I): 0 / Redundancy: 4.2 % / Rmerge(I) obs: 0.073 / Net I/σ(I): 39.8 |
Reflection shell | Resolution: 2.5→2.54 Å / Mean I/σ(I) obs: 2.3 / % possible all: 74.9 |
Reflection | *PLUS Highest resolution: 2.5 Å / Lowest resolution: 40 Å / Num. measured all: 344637 / Rmerge(I) obs: 0.073 |
Reflection shell | *PLUS % possible obs: 74.9 % / Rmerge(I) obs: 0.319 / Mean I/σ(I) obs: 2.3 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1G3I Resolution: 2.5→40 Å / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Solvent model: DENSITY MODIFICATION | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.37 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.5→40 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.5→2.52 Å / Total num. of bins used: 20
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Refinement | *PLUS Num. reflection obs: 71454 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |