+
Open data
-
Basic information
Entry | Database: PDB / ID: 1g41 | ||||||
---|---|---|---|---|---|---|---|
Title | CRYSTAL STRUCTURE OF HSLU HAEMOPHILUS INFLUENZAE | ||||||
![]() | HEAT SHOCK PROTEIN HSLU | ||||||
![]() | CHAPERONE / AAA-ATPASE / CLPY / ATP-DEPENDENT PROTEOLYSIS | ||||||
Function / homology | ![]() HslUV protease complex / proteasome-activating activity / protein unfolding / proteolysis involved in protein catabolic process / peptidase activity / ATP hydrolysis activity / ATP binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Trame, C.B. / McKay, D.B. | ||||||
![]() | ![]() Title: Structure of Haemophilus influenzae HslU protein in crystals with one-dimensional disorder twinning. Authors: Trame, C.B. / McKay, D.B. #1: ![]() Title: Crystal and Solution Structures of an HslUV Protease-Chaperone Complex Authors: Sousa, M.C. / Trame, C.B. / Tsuruta, H. / Wilbanks, S.M. / Reddy, V.S. / McKay, D.B. #2: ![]() Title: The Structures of HslU and the ATP-dependent Protease HslU-HslV Authors: Bochtler, M. / Hartmann, C. / Song, H.K. / Bourenkov, G.P. / Bartunik, H.D. / Huber, R. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 77.3 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 56.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 846.1 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 857.4 KB | Display | |
Data in XML | ![]() | 16.2 KB | Display | |
Data in CIF | ![]() | 22.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1im2C ![]() 1do0S S: Starting model for refinement C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| x 6||||||||
Unit cell |
| ||||||||
Components on special symmetry positions |
| ||||||||
Details | The biological assembly is a hexamer generated from a protomer in the asymmetric unit by the operations: -Y,X-Y,Z and Y-X,-X,Z and -X,-Y,Z and Y,Y-X,Z and X-Y,X,Z |
-
Components
#1: Protein | Mass: 49441.504 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
---|---|
#2: Chemical | ChemComp-SO4 / |
#3: Chemical | ChemComp-ADP / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal grow | Temperature: 291 K / Method: vapor diffusion / pH: 7.3 Details: PEGMME 2000, lithium sulphate, MPD, magnesium sulphate, ADP, pH 7.3, VAPOR DIFFUSION, temperature 291.0K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | *PLUS Temperature: 291 K / pH: 7 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Mar 3, 2000 / Details: double crystal monochromator, mirrors |
Radiation | Monochromator: Double-Crystal Si 111 crystals / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→35.4 Å / Num. all: 53223 / Num. obs: 53223 / % possible obs: 96.5 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.17 % / Biso Wilson estimate: 11.5 Å2 / Rmerge(I) obs: 0.058 / Rsym value: 5.8 / Net I/σ(I): 15.3 |
Reflection shell | Resolution: 2.3→2.34 Å / Redundancy: 2.7 % / Rmerge(I) obs: 0.124 / Mean I/σ(I) obs: 8.5 / Num. unique all: 2563 / Rsym value: 12.4 / % possible all: 95.3 |
Reflection | *PLUS Num. measured all: 168887 |
Reflection shell | *PLUS Highest resolution: 2.3 Å / % possible obs: 95.3 % |
-
Processing
Software |
| |||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1DO0 Resolution: 2.3→35.4 Å / Rfactor Rfree error: 0.004 / Data cutoff high rms absF: 250583.9 / Isotropic thermal model: group / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber Details: Twinning in the crystal produces the P 622 space group and an extended unit cell. Refinement was performed in this setting for one molecule in the asymmetric unit. Please see journal ...Details: Twinning in the crystal produces the P 622 space group and an extended unit cell. Refinement was performed in this setting for one molecule in the asymmetric unit. Please see journal citation for additional details.
| |||||||||||||||||||||||||||||||||
Solvent computation | Solvent model: flat model / Bsol: 36.3531 Å2 / ksol: 0.33177 e/Å3 | |||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 46.2 Å2
| |||||||||||||||||||||||||||||||||
Refine analyze |
| |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.3→35.4 Å
| |||||||||||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 2.3→2.44 Å / Rfactor Rfree error: 0.014 / Total num. of bins used: 6
| |||||||||||||||||||||||||||||||||
Xplor file |
| |||||||||||||||||||||||||||||||||
Software | *PLUS Name: CNS / Version: 1 / Classification: refinement | |||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.3 Å / Lowest resolution: 35.4 Å / σ(F): 0 / % reflection Rfree: 10.1 % | |||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 46.2 Å2 | |||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
| |||||||||||||||||||||||||||||||||
LS refinement shell | *PLUS Highest resolution: 2.3 Å / Rfactor Rfree: 0.394 / % reflection Rfree: 10 % / Rfactor Rwork: 0.387 / Rfactor obs: 0.387 |