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Open data
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Basic information
Entry | Database: PDB / ID: 1.0E+94 | |||||||||
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Title | HslV-HslU from E.coli | |||||||||
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![]() | CHAPERONE / HSLVU / CLPQY / AAA-ATPASE / ATP-DEPENDENT PROTEOLYSIS / PROTEASOME | |||||||||
Function / homology | ![]() peptidase activity => GO:0008233 / HslU-HslV peptidase / protein denaturation / HslUV protease complex / proteasome-activating activity / proteasome core complex / protein unfolding / threonine-type endopeptidase activity / proteolysis involved in protein catabolic process / peptidase activity ...peptidase activity => GO:0008233 / HslU-HslV peptidase / protein denaturation / HslUV protease complex / proteasome-activating activity / proteasome core complex / protein unfolding / threonine-type endopeptidase activity / proteolysis involved in protein catabolic process / peptidase activity / cellular response to heat / response to heat / protein domain specific binding / magnesium ion binding / ATP hydrolysis activity / proteolysis / ATP binding / identical protein binding / membrane / cytoplasm / cytosol Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Song, H.K. / Hartmann, C. / Ravishankar, R. / Bochtler, M. | |||||||||
![]() | ![]() Title: Mutational Studies on Hslu and its Docking Mode with Hslv Authors: Song, H.K. / Hartmann, C. / Ravishankar, R. / Bochtler, M. / Behrendt, R. / Moroder, L. / Huber, R. #1: ![]() Title: The Structures of Hslu and the ATP-Dependent Protease Hslu-Hslv Authors: Bochtler, M. / Hartmann, C. / Song, H.K. / Bourenkov, G.P. / Bartunik, H.D. / Huber, R. #2: ![]() Title: Crystal Structure of Heat Shock Locus V (Hslv) from Escherichia Coli Authors: Bochtler, M. / Ditzel, L. / Groll, M. / Huber, R. #3: Journal: Proc.Natl.Acad.Sci.USA / Year: 1996 Title: Hslv-Hslu: A Novel ATP-Dependent Protease Complex in Escherichia Coli Related to the Eukaryotic Proteasome Authors: Rohrwild, M. / Coux, O. / Huang, H.C. / Moerschell, R.P. / Yoo, S.J. / Seol, J.H. / Chung, C.H. / Goldberg, A.L. #4: Journal: Gene / Year: 1993 Title: Sequence Analysis of Four New Heat-Shock Genes Constituting the Hslts/Ibpab and Hslvu Operons in Escherichia Coli Authors: Chuang, S.E. / Burland, V. / Plunkett III, G. / Daniels, D.L. / Blattner, F.R. | |||||||||
History |
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Remark 700 | SHEET DETERMINATION METHOD: AUTHOR PROVIDED. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 310.6 KB | Display | ![]() |
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PDB format | ![]() | 248.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.2 MB | Display | |
Data in XML | ![]() | 65.4 KB | Display | |
Data in CIF | ![]() | 88.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1doo S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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3 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 18986.641 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Protein | Mass: 50495.531 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #3: Chemical | #4: Water | ChemComp-HOH / | Compound details | EDMAN-DEGRADATION HAS SHOWN THAT THE AMINO-TERMINAL METHIONINE IS CLEAVED IN HSLV TO EXPOSE A ...EDMAN-DEGRADATIO | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.34 Å3/Da / Density % sol: 63.15 % | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 6.3 Details: 20 MG/ML SOLUTION OF HSLU SUPPLEMENTED WITH 1 MM AMP-PNP IN BUFFER (20 MM TRIS/HCL, PH 7.5, 1 MM EDTA, 1 MM NAN3) MIXED IN 2:1 VOLUME RATIO WITH 16 MG/ML HSLV IN 300 MM NACL, 20 MM TRIS/HCL, ...Details: 20 MG/ML SOLUTION OF HSLU SUPPLEMENTED WITH 1 MM AMP-PNP IN BUFFER (20 MM TRIS/HCL, PH 7.5, 1 MM EDTA, 1 MM NAN3) MIXED IN 2:1 VOLUME RATIO WITH 16 MG/ML HSLV IN 300 MM NACL, 20 MM TRIS/HCL, PH 7.5, 1 MM EDTA, 1 MM NAN3. 0.002 ML RESERVOIR PLUS 0.002 ML PROTEIN SOLUTION EQUILIBRATED AGAINST 0.5 ML RESERVOIR SOLUTION. RESERVOIR CONTAINED 100 MM MES, PH 6.3 AND 2.0 M SODIUM ACETATE 0.4 MG/ML RESORUFIN-LABELLED CASEIN | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7.5 / Method: vapor diffusion, sitting dropDetails: A:B=2:1 ratio, Bochtler, M., (2000) Nature, 403, 800. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Jun 1, 2000 / Details: MIRROR |
Radiation | Monochromator: DOUBLE CRYSTAL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0712 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→25 Å / Num. obs: 59863 / % possible obs: 99.8 % / Redundancy: 11.8 % / Rmerge(I) obs: 0.12 / Rsym value: 0.12 |
Reflection shell | Resolution: 2.8→2.87 Å / % possible all: 99.8 |
Reflection | *PLUS Highest resolution: 2.8 Å / Lowest resolution: 25 Å / Num. measured all: 706383 / Rmerge(I) obs: 0.12 |
Reflection shell | *PLUS % possible obs: 99.8 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1DOO ![]() 1doo Resolution: 2.8→15 Å / Cross valid method: THROUGHOUT / σ(F): 0 Details: ZERO OCCUPANCY COORDINATES IN I- DOMAIN ARE TAKEN FROM THOSE OF TRIGONAL HSLU MODEL (1DO2) THE ELECTRON DENSITY OF RESIDUES FROM 175 - 209 IN HSLU MODEL (CHAIN E AND F) IS COMPLETELY ...Details: ZERO OCCUPANCY COORDINATES IN I- DOMAIN ARE TAKEN FROM THOSE OF TRIGONAL HSLU MODEL (1DO2) THE ELECTRON DENSITY OF RESIDUES FROM 175 - 209 IN HSLU MODEL (CHAIN E AND F) IS COMPLETELY DISORDERED THE ELECTRON DENSITY OF CORE REGION (RESIDUE FROM 89 - 92) IN HSLU (CHAIN E AND F) WAS NOT CLEAR AND MANY ATOMS IN THIS REGION HAVE ZERO OCCUPANCY.
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Solvent computation | Solvent model: FLAT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.8→15 Å
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Refine LS restraints |
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Xplor file |
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Software | *PLUS Name: CNS / Version: 1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor Rfree: 0.3044 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: c_bond_d / Dev ideal: 0.0142 |