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- PDB-1oau: Fv Structure of the IgE SPE-7 in complex with DNP-Ser (immunising... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1oau | ||||||
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Title | Fv Structure of the IgE SPE-7 in complex with DNP-Ser (immunising hapten) | ||||||
![]() | (IMMUNOGLOBULIN ...) x 2 | ||||||
![]() | IMMUNE SYSTEM / ANTIBODY-COMPLEX / ANTIBODY / ALLERGY / IGE / CONFORMATIONAL DIVERSITY / MULTISPECIFICITY | ||||||
Function / homology | ![]() immunoglobulin complex / adaptive immune response / immune response / extracellular space Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | James, L.C. / Roversi, P. / Tawfik, D. | ||||||
![]() | ![]() Title: Antibody Multispecificity Mediated by Conformational Diversity Authors: James, L.C. / Roversi, P. / Tawfik, D. | ||||||
History |
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Remark 700 | SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN ... SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 149 KB | Display | ![]() |
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PDB format | ![]() | 123.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 533 KB | Display | ![]() |
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Full document | ![]() | 560 KB | Display | |
Data in XML | ![]() | 34.1 KB | Display | |
Data in CIF | ![]() | 46.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1oaqC ![]() 1oarC ![]() 1oaxC ![]() 1oayC ![]() 1oazC ![]() 1ocwC ![]() 1anqS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
-Antibody , 2 types, 8 molecules HIJKLMNO
#1: Antibody | Mass: 13687.315 Da / Num. of mol.: 4 / Fragment: FV REGION, RESIDUES 1-122 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Antibody | Mass: 11530.808 Da / Num. of mol.: 4 / Fragment: FV REGION, RESIDUES 1-110 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Non-polymers , 4 types, 401 molecules ![](data/chem/img/SER.gif)
![](data/chem/img/DNF.gif)
![](data/chem/img/IMD.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/DNF.gif)
![](data/chem/img/IMD.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | #4: Chemical | #5: Chemical | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.5 Å3/Da / Density % sol: 70 % | ||||||||||||||||||||||||
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Crystal grow | pH: 7 Details: 24% PEG8K,0.1M NA ACETATE, 0.2M AMMONIUM SULPHATE, PH7, pH 7.00 | ||||||||||||||||||||||||
Crystal grow | *PLUS pH: 5.6 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Sep 15, 2001 / Details: MIRRORS |
Radiation | Monochromator: NI FILTER / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.807→30.015 Å / Num. obs: 90268 / % possible obs: 91.8 % / Observed criterion σ(I): 2 / Redundancy: 3 % / Rsym value: 0.058 / Net I/σ(I): 10.9 |
Reflection shell | Resolution: 1.81→1.9 Å / Redundancy: 1.8 % / Mean I/σ(I) obs: 1.2 / Rsym value: 0.399 / % possible all: 70.3 |
Reflection | *PLUS Highest resolution: 1.8 Å / Num. obs: 90267 / Redundancy: 3 % / Rmerge(I) obs: 0.07 |
Reflection shell | *PLUS % possible obs: 91.4 % / Rmerge(I) obs: 0.235 / Mean I/σ(I) obs: 1.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1ANQ Resolution: 1.8→30.02 Å / Cor.coef. Fo:Fc: 0.93 / Cor.coef. Fo:Fc free: 0.915 / SU B: 5.653 / SU ML: 0.149 / Cross valid method: THROUGHOUT / ESU R: 0.071 / ESU R Free: 0.206 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL PLUS MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 18.64 Å2
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Refinement step | Cycle: LAST / Resolution: 1.8→30.02 Å
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Refine LS restraints |
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