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Open data
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Basic information
Entry | Database: PDB / ID: 1o05 | ||||||
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Title | Apo form of human mitochondrial aldehyde dehydrogenase | ||||||
![]() | Aldehyde dehydrogenase | ||||||
![]() | OXIDOREDUCTASE / ALDH / NAD / NADH / isomerization | ||||||
Function / homology | ![]() Metabolism of serotonin / : / regulation of dopamine biosynthetic process / regulation of serotonin biosynthetic process / aldehyde catabolic process / phenylacetaldehyde dehydrogenase (NAD+) activity / alcohol metabolic process / aldehyde dehydrogenase [NAD(P)+] activity / ethanol catabolic process / Ethanol oxidation ...Metabolism of serotonin / : / regulation of dopamine biosynthetic process / regulation of serotonin biosynthetic process / aldehyde catabolic process / phenylacetaldehyde dehydrogenase (NAD+) activity / alcohol metabolic process / aldehyde dehydrogenase [NAD(P)+] activity / ethanol catabolic process / Ethanol oxidation / carboxylesterase activity / glyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity / aldehyde dehydrogenase (NAD+) / aldehyde dehydrogenase (NAD+) activity / Smooth Muscle Contraction / Mitochondrial protein degradation / NAD binding / carbohydrate metabolic process / electron transfer activity / mitochondrial matrix / mitochondrion / extracellular exosome Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Hurley, T.D. / Perez-Miller, S. / Breen, H. | ||||||
![]() | ![]() Title: Order and disorder in mitochondrial aldehyde dehydrogenase Authors: Hurley, T.D. / Perez-Miller, S. / Breen, H. #1: ![]() Title: Coenzyme isomerization is integral to catalysis in aldehyde dehydrogenase Authors: Perez-Miller, S.J. / Hurley, T.D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 803.6 KB | Display | ![]() |
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PDB format | ![]() | 660.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1cw3S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 54499.629 Da / Num. of mol.: 8 Fragment: Complete mature sequence (does not contain mitochondrial leader sequence). Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-NA / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.02 Å3/Da / Density % sol: 38.6 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.4 Details: ACES, PEG 6000, Guanidine HCl, MgCl2, DTT., pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 6.2 / Method: unknown / Details: Ni, L., (1999) Protein Sci., 8, 2784. | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE / Date: Nov 3, 1999 |
Radiation | Monochromator: Yale mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.25→25 Å / Num. all: 181352 / Num. obs: 165756 / % possible obs: 91.4 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3 % / Biso Wilson estimate: 12.6 Å2 / Rmerge(I) obs: 0.072 / Net I/σ(I): 14.3 |
Reflection shell | Resolution: 2.25→2.33 Å / Rmerge(I) obs: 0.214 / Mean I/σ(I) obs: 3.5 / Num. unique all: 11115 / % possible all: 62 |
Reflection | *PLUS Lowest resolution: 42 Å / Num. measured all: 500412 |
Reflection shell | *PLUS % possible obs: 62 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1CW3 Resolution: 2.25→24.96 Å / Rfactor Rfree error: 0.002 / Data cutoff high absF: 5593690.14 / Data cutoff high rms absF: 5593690.14 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber / Details: maximum likelihood target using amplitudes
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 10 Å2 / ksol: 0.299682 e/Å3 | ||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 16.8 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.25→24.96 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.25→2.33 Å / Rfactor Rfree error: 0.011 / Total num. of bins used: 10
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Xplor file |
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Refinement | *PLUS Lowest resolution: 42 Å / Rfactor Rfree: 0.226 / Rfactor Rwork: 0.176 | ||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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