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Yorodumi- PDB-1m9l: Relaxation-based Refined Structure Of Chlamydomonas Outer Arm Dyn... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1m9l | ||||||
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Title | Relaxation-based Refined Structure Of Chlamydomonas Outer Arm Dynein Light Chain 1 | ||||||
Components | Outer Arm Dynein Light Chain 1 | ||||||
Keywords | CONTRACTILE PROTEIN / Leucine-rich repeat / Relaxation / Structural refinement / Backbone dynamics / Structure from MOLMOL | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Chlamydomonas reinhardtii (plant) | ||||||
Method | SOLUTION NMR / simulated annealing, molecular dynamics | ||||||
Authors | Wu, H.W. / Maciejewski, M.W. / Marintchev, A. / Benashski, S.E. / Mullen, G.P. / King, S.M. | ||||||
Citation | Journal: Biochemistry / Year: 2003 Title: Relaxation-based structure refinement and backbone molecular dynamics of the Dynein motor domain-associated light chain Authors: Wu, H. / Blackledge, M. / Maciejewski, M.W. / Mullen, G.P. / King, S.M. #1: Journal: Nat.Struct.Biol. / Year: 2000 Title: Solution Structure of A Dynein Motor Domain Associated Light Chain Authors: Wu, H.W. / Maciejewski, M.W. / Marintchev, A. / Benashski, S.E. / Mullen, G.P. / King, S.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1m9l.cif.gz | 916 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1m9l.ent.gz | 791.9 KB | Display | PDB format |
PDBx/mmJSON format | 1m9l.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/m9/1m9l ftp://data.pdbj.org/pub/pdb/validation_reports/m9/1m9l | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 22317.715 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Chlamydomonas reinhardtii (plant) / Plasmid: pET16b / Production host: Escherichia coli (E. coli) / Strain (production host): 1132D(-) / References: UniProt: Q9XHH2 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: using relaxation R2/R1 ratios as long range constraints to refine structure. |
-Sample preparation
Details | Contents: ~2.0 mM Light Chain 1 protein, U-15N, 2.5 mM Tris.Cl pH 6.7, 100 mM NaCl; 90% H2O, 10% D2O. Solvent system: 90% H2O/10% D2O |
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Sample conditions | pH: 6.7 / Pressure: ambient / Temperature: 298 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing, molecular dynamics / Software ordinal: 1 | ||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||
NMR ensemble | Conformer selection criteria: all calculated structures submitted Conformers calculated total number: 15 / Conformers submitted total number: 15 |