[English] 日本語
Yorodumi- PDB-1ds9: SOLUTION STRUCTURE OF CHLAMYDOMONAS OUTER ARM DYNEIN LIGHT CHAIN 1 -
+Open data
-Basic information
Entry | Database: PDB / ID: 1ds9 | ||||||
---|---|---|---|---|---|---|---|
Title | SOLUTION STRUCTURE OF CHLAMYDOMONAS OUTER ARM DYNEIN LIGHT CHAIN 1 | ||||||
Components | OUTER ARM DYNEIN | ||||||
Keywords | CONTRACTILE PROTEIN / LEUCINE-RICH REPEAT / BETA-BETA-ALPHA CYLINDER / DYNEIN / CHLAMYDOMONAS / FLAGELLA | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Chlamydomonas reinhardtii (plant) | ||||||
Method | SOLUTION NMR / simulated annealing, torsion angle dynamics | ||||||
Authors | Wu, H.W. / Maciejewski, M.W. / Marintchev, A. / Benashski, S.E. / Mullen, G.P. / King, S.M. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 2000 Title: Solution structure of a dynein motor domain associated light chain. Authors: Wu, H. / Maciejewski, M.W. / Marintchev, A. / Benashski, S.E. / Mullen, G.P. / King, S.M. #1: Journal: J.Biomol.NMR / Year: 1999 Title: 1H, 15N and 13C resonance assignments for the 22 kDa LC1 light chain from Chlamydomonas outer arm dynein Authors: Wu, H. / Maciejewski, M.W. / Benashski, S.E. / Mullen, G.P. / King, S.M. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1ds9.cif.gz | 1 MB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1ds9.ent.gz | 897.5 KB | Display | PDB format |
PDBx/mmJSON format | 1ds9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ds/1ds9 ftp://data.pdbj.org/pub/pdb/validation_reports/ds/1ds9 | HTTPS FTP |
---|
-Related structure data
Similar structure data | |
---|---|
Other databases |
|
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 22179.570 Da / Num. of mol.: 1 / Fragment: LIGHT CHAIN 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Chlamydomonas reinhardtii (plant) / Organ: FLAGELLAE / Plasmid: PET16B / Production host: Escherichia coli (E. coli) / References: UniProt: Q9XHH2 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
---|---|
NMR experiment | Type: 3D 13C edited NOESY |
NMR details | Text: NMR experimental data are described in BIOMAGRESBANK deposition #4265 |
-Sample preparation
Details |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Sample conditions | Ionic strength: 100 mM NaCl / pH: 6.76 / Pressure: ambient / Temperature: 298 K | |||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
|
---|
-Processing
NMR software |
| ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: simulated annealing, torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations,structures with the lowest energy,target function Conformers calculated total number: 150 / Conformers submitted total number: 17 |