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- PDB-1m4j: CRYSTAL STRUCTURE OF THE N-TERMINAL ADF-H DOMAIN OF MOUSE TWINFIL... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1m4j | ||||||
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Title | CRYSTAL STRUCTURE OF THE N-TERMINAL ADF-H DOMAIN OF MOUSE TWINFILIN ISOFORM-1 | ||||||
![]() | A6 gene product | ||||||
![]() | STRUCTURAL PROTEIN / MIXED BETA-SHEET / PAIR OF ALPHA-HELICES | ||||||
Function / homology | ![]() regulation of actin phosphorylation / RHOBTB2 GTPase cycle / sequestering of actin monomers / negative regulation of actin filament polymerization / actin filament depolymerization / barbed-end actin filament capping / regulation of lamellipodium assembly / myofibril / positive regulation of cardiac muscle hypertrophy / actin monomer binding ...regulation of actin phosphorylation / RHOBTB2 GTPase cycle / sequestering of actin monomers / negative regulation of actin filament polymerization / actin filament depolymerization / barbed-end actin filament capping / regulation of lamellipodium assembly / myofibril / positive regulation of cardiac muscle hypertrophy / actin monomer binding / phosphatidylinositol-4,5-bisphosphate binding / filopodium / actin filament / positive regulation of neuron projection development / cell-cell junction / actin filament binding / actin cytoskeleton / protein tyrosine kinase activity / protein-containing complex binding / perinuclear region of cytoplasm / ATP binding / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Paavilainen, V.O. / Merckel, M.C. / Falck, S. / Ojala, P.J. / Pohl, E. / Wilmanns, M. / Lappalainen, P. | ||||||
![]() | ![]() Title: Structural Conservation Between the Actin Monomer-binding Sites of Twinfilin and Actin-depolymerizing Factor (ADF)/Cofilin Authors: Paavilainen, V.O. / Merckel, M.C. / Falck, S. / Ojala, P.J. / Pohl, E. / Wilmanns, M. / Lappalainen, P. #1: ![]() Title: Twinfilin Forms a Stable Complex with ADP-Actin Monomers Through its Carboxyl-Terminal ADF-H Domain Authors: Ojala, P.J. / Paavilainen, V.O. / Vartiainen, M.K. / Tuma, R. / Weeds, A.G. / Lappalainen, P. #2: ![]() Title: Mouse A6/twinfilin is an actin monomer-binding protein that localizes to the regions of rapid actin dynamics Authors: Vartiainen, M. / Ojala, P.J. / Auvinen, P. / Peranen, J. / Lappalainen, P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 65.3 KB | Display | ![]() |
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PDB format | ![]() | 49.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 437.6 KB | Display | ![]() |
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Full document | ![]() | 443.4 KB | Display | |
Data in XML | ![]() | 13.2 KB | Display | |
Data in CIF | ![]() | 17.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 16294.358 Da / Num. of mol.: 2 / Fragment: N-terminal ADF-H Domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.09 Å3/Da / Density % sol: 41.14 % | |||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 1.6M Na-Citrate, 3% PEG 400, 0.1M Na-Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K | |||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ | |||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Nov 23, 2001 / Details: Premirrors, bent mirror |
Radiation | Monochromator: Triangular monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8459 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→20 Å / Num. all: 36771 / Num. obs: 36771 / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.8 % / Biso Wilson estimate: 21.1 Å2 / Rmerge(I) obs: 0.044 / Rsym value: 0.044 |
Reflection shell | Resolution: 1.6→1.7 Å / Mean I/σ(I) obs: 4 / Num. unique all: 3101 / Rsym value: 0.255 / % possible all: 87.5 |
Reflection | *PLUS Highest resolution: 1.6 Å / Lowest resolution: 20 Å / Num. obs: 34257 / % possible obs: 93 % / Num. measured all: 457961 |
Reflection shell | *PLUS Lowest resolution: 1.66 Å / % possible obs: 92.2 % / Rmerge(I) obs: 0.255 |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso mean: 21.5 Å2 | |||||||||||||||||||||||||
Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.6→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.6→1.7 Å / Rfactor Rfree error: 0.012 / Total num. of bins used: 6
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Refinement | *PLUS Lowest resolution: 20 Å / % reflection Rfree: 9 % / Rfactor Rfree: 0.251 / Rfactor Rwork: 0.232 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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