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Open data
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Basic information
Entry | Database: PDB / ID: 1cfy | ||||||
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Title | YEAST COFILIN, MONOCLINIC CRYSTAL FORM | ||||||
![]() | COFILIN | ||||||
![]() | ACTIN-BINDING PROTEIN / ACTIN-BINDING / CYTOSKELETON | ||||||
Function / homology | ![]() actin filament fragmentation / actin cortical patch / actin filament severing / Golgi to plasma membrane protein transport / actin filament depolymerization / actin filament organization / nuclear matrix / endocytosis / actin filament binding / actin cytoskeleton ...actin filament fragmentation / actin cortical patch / actin filament severing / Golgi to plasma membrane protein transport / actin filament depolymerization / actin filament organization / nuclear matrix / endocytosis / actin filament binding / actin cytoskeleton / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Fedorov, A.A. / Lappalainen, P. / Fedorov, E.V. / Drubin, D.G. / Almo, S.C. | ||||||
![]() | ![]() Title: Structure determination of yeast cofilin. Authors: Fedorov, A.A. / Lappalainen, P. / Fedorov, E.V. / Drubin, D.G. / Almo, S.C. #1: ![]() Title: The Adf/Cofilin Proteins: Stimulus-Responsive Modulators of Actin Dynamics Authors: Moon, A. / Drubin, D.G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 63.2 KB | Display | ![]() |
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PDB format | ![]() | 47.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 369.6 KB | Display | ![]() |
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Full document | ![]() | 372.6 KB | Display | |
Data in XML | ![]() | 6.4 KB | Display | |
Data in CIF | ![]() | 9.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1cofSC ![]() 1qpvC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.185263, -0.000637, -0.982689), Vector: |
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Components
#1: Protein | Mass: 15919.742 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.82 Å3/Da / Density % sol: 57 % |
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Crystal grow | pH: 7.2 / Details: CRYSTALLIZED FROM 30% PEG 4000 SOLUTION., pH 7.2 |
Crystal grow | *PLUS Method: vapor diffusion, hanging drop / PH range low: 7.5 / PH range high: 7 |
Components of the solutions | *PLUS Conc.: 20-30 % / Common name: PEG4000 |
-Data collection
Diffraction | Mean temperature: 290 K |
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Diffraction source | Source: ![]() |
Detector | Type: SIEMENS / Detector: AREA DETECTOR / Date: 1995 |
Radiation | Monochromator: GRAPHITE(002) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→21.6 Å / Num. obs: 13627 / % possible obs: 82.8 % / Observed criterion σ(I): 2 / Redundancy: 1.7 % / Rmerge(I) obs: 0.051 / Net I/σ(I): 19.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1COF Resolution: 2.3→8 Å / σ(F): 2
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Displacement parameters | Biso mean: 30.5 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.23 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.3→8 Å
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Refine LS restraints |
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Xplor file |
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