+Open data
-Basic information
Entry | Database: PDB / ID: 1cof | ||||||
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Title | YEAST COFILIN, ORTHORHOMBIC CRYSTAL FORM | ||||||
Components | COFILIN | ||||||
Keywords | ACTIN-BINDING PROTEIN / ACTIN-BINDING / CYTOSKELETON | ||||||
Function / homology | Function and homology information actin filament fragmentation / actin cortical patch / actin filament severing / Golgi to plasma membrane protein transport / actin filament depolymerization / actin filament organization / nuclear matrix / endocytosis / actin filament binding / actin cytoskeleton ...actin filament fragmentation / actin cortical patch / actin filament severing / Golgi to plasma membrane protein transport / actin filament depolymerization / actin filament organization / nuclear matrix / endocytosis / actin filament binding / actin cytoskeleton / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae (brewer's yeast) | ||||||
Method | X-RAY DIFFRACTION / MIR / Resolution: 2.3 Å | ||||||
Authors | Fedorov, A.A. / Lappalainen, P. / Fedorov, E.V. / Drubin, D.G. / Almo, S.C. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 1997 Title: Structure determination of yeast cofilin. Authors: Fedorov, A.A. / Lappalainen, P. / Fedorov, E.V. / Drubin, D.G. / Almo, S.C. #1: Journal: Mol.Cell.Biol. / Year: 1995 Title: The Adf/Cofilin Proteins: Stimulus-Responsive Modulators of Actin Dynamics Authors: Moon, A. / Drubin, D.G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1cof.cif.gz | 37.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1cof.ent.gz | 26.1 KB | Display | PDB format |
PDBx/mmJSON format | 1cof.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1cof_validation.pdf.gz | 363.1 KB | Display | wwPDB validaton report |
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Full document | 1cof_full_validation.pdf.gz | 364.1 KB | Display | |
Data in XML | 1cof_validation.xml.gz | 4 KB | Display | |
Data in CIF | 1cof_validation.cif.gz | 5.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/co/1cof ftp://data.pdbj.org/pub/pdb/validation_reports/co/1cof | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 15919.742 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast) Production host: Escherichia coli (E. coli) / References: UniProt: Q03048 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.97 Å3/Da / Density % sol: 38 % |
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Crystal grow | pH: 7.2 / Details: CRYSTALLIZED FROM 25% PEG 4000 SOLUTION., pH 7.2 |
Crystal grow | *PLUS Method: vapor diffusion, hanging drop / PH range low: 7.5 / PH range high: 7 |
Components of the solutions | *PLUS Conc.: 20-30 % / Common name: PEG4000 |
-Data collection
Diffraction | Mean temperature: 290 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RUH2R / Wavelength: 1.5418 |
Detector | Type: SIEMENS / Detector: AREA DETECTOR / Date: 1995 |
Radiation | Monochromator: GRAPHITE(002) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→26.2 Å / Num. obs: 6330 / % possible obs: 87.5 % / Observed criterion σ(I): 2 / Redundancy: 2.33 % / Rmerge(I) obs: 0.049 / Net I/σ(I): 18.5 |
Reflection | *PLUS Rmerge(I) obs: 0.067 |
-Processing
Software |
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Refinement | Method to determine structure: MIR / Resolution: 2.3→8 Å / σ(F): 2 Details: N-TERMINAL RESIDUES 1 - 5 AND C-TERMINAL RESIDUES 141 - 143 ARE NOT VISIBLE IN THE MAPS. LOOP 73 - 78 HAS WEAK ELECTRON DENSITY.
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Displacement parameters | Biso mean: 25.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.23 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.3→8 Å
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Refine LS restraints |
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Xplor file |
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Software | *PLUS Name: PROFFT / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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