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Open data
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Basic information
Entry | Database: PDB / ID: 3uh6 | ||||||
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Title | HBI (T72A) CO bound | ||||||
![]() | Globin-1 | ||||||
![]() | OXYGEN STORAGE / OXYGEN TRANSPORT / allostery / oxygen binding | ||||||
Function / homology | ![]() oxygen carrier activity / oxygen binding / response to hypoxia / heme binding / identical protein binding / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Ren, Z. / Srajer, V. / Knapp, J.E. / Royer Jr., W.E. | ||||||
![]() | ![]() Title: Cooperative macromolecular device revealed by meta-analysis of static and time-resolved structures. Authors: Ren, Z. / Srajer, V. / Knapp, J.E. / Royer, W.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 67.4 KB | Display | ![]() |
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PDB format | ![]() | 50.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 3qobC ![]() 3ugyC ![]() 3ugzC ![]() 3uh3C ![]() 3uh5C ![]() 3uh7C ![]() 3uhbC ![]() 3uhcC ![]() 3uhdC ![]() 3uheC ![]() 3uhgC ![]() 3uhhC ![]() 3uhiC ![]() 3uhkC ![]() 3uhnC ![]() 3uhqC ![]() 3uhrC ![]() 3uhsC ![]() 3uhtC ![]() 3uhuC ![]() 3uhvC ![]() 3uhwC ![]() 3uhxC ![]() 3uhyC ![]() 3uhzC ![]() 3ui0C ![]() 3sdhS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 15937.276 Da / Num. of mol.: 2 / Mutation: T72A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.27 Å3/Da / Density % sol: 45.7 % |
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Crystal grow | Temperature: 298 K / Method: small tubes / pH: 7.5 / Details: phosphate, pH 7.5, SMALL TUBES, temperature 298K |
-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE / Date: Jun 27, 2001 |
Radiation | Monochromator: YALE MIRRORS / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.25→40 Å / Num. all: 13787 / Num. obs: 13167 / % possible obs: 95.5 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3 % / Biso Wilson estimate: 11.4 Å2 / Rmerge(I) obs: 0.084 / Rsym value: 0.084 / Net I/σ(I): 11.2 |
Reflection shell | Resolution: 2.25→2.33 Å / Rmerge(I) obs: 0.202 / Mean I/σ(I) obs: 3.2 / Rsym value: 0.202 / % possible all: 90.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 3SDH Resolution: 2.25→30.15 Å / Rfactor Rfree error: 0.009 / Data cutoff high absF: 1619275.32 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber / Details: BULK SOLVENT MODEL USED
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 33.2848 Å2 / ksol: 0.35 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 20.5 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.25→30.15 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.25→2.39 Å / Rfactor Rfree error: 0.024 / Total num. of bins used: 6
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Xplor file |
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