+
Open data
-
Basic information
Entry | Database: PDB / ID: 3ugz | ||||||
---|---|---|---|---|---|---|---|
Title | HBI (L36A) CO bound | ||||||
![]() | Globin-1 | ||||||
![]() | OXYGEN STORAGE / OXYGEN TRANSPORT / allostery / oxygen binding | ||||||
Function / homology | ![]() oxygen carrier activity / oxygen binding / response to hypoxia / heme binding / identical protein binding / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Ren, Z. / Srajer, V. / Knapp, J.E. / Royer Jr., W.E. | ||||||
![]() | ![]() Title: Cooperative macromolecular device revealed by meta-analysis of static and time-resolved structures. Authors: Ren, Z. / Srajer, V. / Knapp, J.E. / Royer, W.E. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 76 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 56.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 15 KB | Display | |
Data in CIF | ![]() | 20.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3qobC ![]() 3ugyC ![]() 3uh3C ![]() 3uh5C ![]() 3uh6C ![]() 3uh7C ![]() 3uhbC ![]() 3uhcC ![]() 3uhdC ![]() 3uheC ![]() 3uhgC ![]() 3uhhC ![]() 3uhiC ![]() 3uhkC ![]() 3uhnC ![]() 3uhqC ![]() 3uhrC ![]() 3uhsC ![]() 3uhtC ![]() 3uhuC ![]() 3uhvC ![]() 3uhwC ![]() 3uhxC ![]() 3uhyC ![]() 3uhzC ![]() 3ui0C ![]() 3sdhS S: Starting model for refinement C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 15925.224 Da / Num. of mol.: 2 / Mutation: L36A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.29 Å3/Da / Density % sol: 46.17 % |
---|---|
Crystal grow | Temperature: 298 K / Method: small tubes / pH: 7.5 Details: sodium/potassium phosphate, pH 7.5, SMALL TUBES, temperature 298K |
-Data collection
Diffraction | Mean temperature: 298 K |
---|---|
Diffraction source | Source: ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Jan 24, 2005 |
Radiation | Monochromator: OSMIC MIRRORS / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.65→40 Å / Num. all: 34987 / Num. obs: 32468 / % possible obs: 92.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.3 % / Biso Wilson estimate: 22.4 Å2 / Rmerge(I) obs: 0.093 / Rsym value: 0.093 / Net I/σ(I): 11.6 |
Reflection shell | Resolution: 1.65→1.71 Å / Rmerge(I) obs: 0.294 / Mean I/σ(I) obs: 3.8 / Rsym value: 0.294 / % possible all: 87.6 |
-
Processing
Software |
| |||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 3SDH Resolution: 1.65→25.09 Å / Rfactor Rfree error: 0.005 / Data cutoff high absF: 1204033.16 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber / Details: BULK SOLVENT MODEL USED
| |||||||||||||||||||||||||
Solvent computation | Solvent model: FLAT MODEL / Bsol: 45.6186 Å2 / ksol: 0.35 e/Å3 | |||||||||||||||||||||||||
Displacement parameters | Biso mean: 19.2 Å2
| |||||||||||||||||||||||||
Refine analyze |
| |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.65→25.09 Å
| |||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||
LS refinement shell | Resolution: 1.65→1.75 Å / Rfactor Rfree error: 0.02 / Total num. of bins used: 6
| |||||||||||||||||||||||||
Xplor file |
|