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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1lfg | |||||||||
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タイトル | Structure of diferric human lactoferrin | |||||||||
![]() | LACTOFERRIN | |||||||||
![]() | TRANSFERRIN | |||||||||
機能・相同性 | ![]() negative regulation by host of viral process / membrane destabilizing activity / Mtb iron assimilation by chelation / phagocytic vesicle lumen / positive regulation of toll-like receptor 4 signaling pathway / Metal sequestration by antimicrobial proteins / negative regulation of viral process / negative regulation of cysteine-type endopeptidase activity / negative regulation of tumor necrosis factor (ligand) superfamily member 11 production / negative regulation of single-species biofilm formation in or on host organism ...negative regulation by host of viral process / membrane destabilizing activity / Mtb iron assimilation by chelation / phagocytic vesicle lumen / positive regulation of toll-like receptor 4 signaling pathway / Metal sequestration by antimicrobial proteins / negative regulation of viral process / negative regulation of cysteine-type endopeptidase activity / negative regulation of tumor necrosis factor (ligand) superfamily member 11 production / negative regulation of single-species biofilm formation in or on host organism / positive regulation of bone mineralization involved in bone maturation / negative regulation of lipopolysaccharide-mediated signaling pathway / specific granule / negative regulation of osteoclast development / antifungal humoral response / positive regulation of chondrocyte proliferation / negative regulation of ATP-dependent activity / regulation of tumor necrosis factor production / bone morphogenesis / Antimicrobial peptides / cysteine-type endopeptidase inhibitor activity / negative regulation of viral genome replication / positive regulation of osteoblast proliferation / humoral immune response / 加水分解酵素; プロテアーゼ; ペプチド結合加水分解酵素; セリンエンドペプチターゼ / positive regulation of osteoblast differentiation / regulation of cytokine production / protein serine/threonine kinase activator activity / ossification / innate immune response in mucosa / secretory granule / lipopolysaccharide binding / positive regulation of protein serine/threonine kinase activity / recycling endosome / specific granule lumen / antimicrobial humoral immune response mediated by antimicrobial peptide / tertiary granule lumen / positive regulation of NF-kappaB transcription factor activity / heparin binding / iron ion transport / antibacterial humoral response / positive regulation of canonical NF-kappaB signal transduction / defense response to Gram-negative bacterium / killing of cells of another organism / early endosome / iron ion binding / Amyloid fiber formation / serine-type endopeptidase activity / Neutrophil degranulation / negative regulation of apoptotic process / cell surface / protein-containing complex / proteolysis / DNA binding / extracellular space / extracellular exosome / extracellular region / nucleus / plasma membrane / cytoplasm 類似検索 - 分子機能 | |||||||||
生物種 | ![]() | |||||||||
手法 | ![]() | |||||||||
![]() | Baker, E.N. / Anderson, B.F. / Haridas, M. | |||||||||
![]() | ![]() タイトル: Structure of human diferric lactoferrin refined at 2.2 A resolution. 著者: Haridas, M. / Anderson, B.F. / Baker, E.N. #1: ![]() タイトル: Structure of Human Lactoferrin: Crystallographic Structure Analysis and Refinement at 2.8 Angstroms Resolution 著者: Anderson, B.F. / Baker, H.M. / Norris, G.E. / Rice, D.W. / Baker, E.N. #2: ![]() タイトル: Transferrins: Insights Into Structure and Function from Studies on Lactoferrin 著者: Baker, E.N. / Rumball, S.V. / Anderson, B.F. #3: ![]() タイトル: Structure of Human Lactoferrin at 3.2 Angstroms Resolution 著者: Anderson, B.F. / Baker, H.M. / Dodson, E.J. / Norris, G.E. / Rumball, S.V. / Waters, J.M. / Baker, E.N. | |||||||||
履歴 |
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Remark 700 | SHEET THERE ARE SEVERAL BIFURCATED SHEETS IN THIS STRUCTURE. THESE ARE REPRESENTED BY TWO SHEETS ...SHEET THERE ARE SEVERAL BIFURCATED SHEETS IN THIS STRUCTURE. THESE ARE REPRESENTED BY TWO SHEETS WHICH HAVE ONE OR MORE IDENTICAL STRANDS. SHEETS *N2A* AND *N2B* REPRESENT ONE BIFURCATED SHEET WHERE STRAND 4 OF SHEET RECORD BN1 IS ALSO PART OF THIS SHEET. SHEETS *C2A* AND *C2B* REPRESENT ONE BIFURCATED SHEET WHERE STRAND 4 OF SHEET RECORD BC1 IS ALSO PART OF THIS SHEET. | |||||||||
Remark 650 | HELIX ON HELIX RECORDS THE TYPES OF DISTORTION IN H BONDING OF N AND C TERMINI CLASSIFIED AS IN F.N. ...HELIX ON HELIX RECORDS THE TYPES OF DISTORTION IN H BONDING OF N AND C TERMINI CLASSIFIED AS IN F.N.BAKER AND R.F.HUBBARD (1984) PROG. BIOPHYS. MOL. BIOL. 44, 97-179. |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 161.1 KB | 表示 | ![]() |
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PDB形式 | ![]() | 124.8 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 518.5 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 577.7 KB | 表示 | |
XML形式データ | ![]() | 23.4 KB | 表示 | |
CIF形式データ | ![]() | 35.9 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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Atom site foot note | 1: CIS PROLINE - PRO 71 / 2: CIS PROLINE - PRO 142 / 3: CIS PROLINE - PRO 628 / 4: RESIDUES 1-4 POORLY DEFINED DENSITY. / 5: RESIDUES 293-294 POORLY DEFINED DENSITY. / 6: RESIDUES 418-422 POORLY DEFINED DENSITY. / 7: ASN 137 CARBOHYDRATE ATTACHMENT SITE. / 8: ASN 478 CARBOHYDRATE ATTACHMENT SITE. 9: NO SIDECHAINS BEYOND CB FOR ARG 86 AND GLU 637 (TREATED AS ALA). |
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要素
-タンパク質 , 1種, 1分子 A
#1: タンパク質 | 分子量: 76079.039 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() |
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-糖 , 2種, 2分子
#2: 多糖 | alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose |
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#3: 多糖 | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta- ...2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose |
-非ポリマー , 3種, 497分子 ![](data/chem/img/FE.gif)
![](data/chem/img/CO3.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CO3.gif)
![](data/chem/img/HOH.gif)
#4: 化合物 | #5: 化合物 | #6: 水 | ChemComp-HOH / | |
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-詳細
構成要素の詳細 | TWO-FOLD INTERNAL SEQUENCE HOMOLOGY (~ 40% IDENTITY). ONE BINDING SITE IN EACH LOBE. | ||
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非ポリマーの詳細 | VERY POOR DENSITY FOR THE CARBOHYDRA配列の詳細 | AMINO ACID SEQUENCE GIVEN IN ANDERSON ET AL. (1989) (COMPOSITE OF CHEMICALLY-DETERMINED AND CDNA ...AMINO ACID SEQUENCE GIVEN IN ANDERSON ET AL. (1989) (COMPOSITE OF CHEMICALLY | |
-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.79 Å3/Da / 溶媒含有率: 55.94 % | ||||||||||||||||||||
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結晶化 | *PLUS 温度: 4 ℃ / pH: 7.8 / 手法: microdialysis詳細: taken from Baker, E.N. et al (1977). J. Mol. Biol., 111, 207-210. | ||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
放射 | 散乱光タイプ: x-ray |
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放射波長 | 相対比: 1 |
反射 | *PLUS Num. obs: 17783 / Rmerge(I) obs: 0.09 |
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解析
ソフトウェア | 名称: PROFFT / 分類: 精密化 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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精密化 | Rfactor obs: 0.183 / 最高解像度: 2.2 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: LAST / 最高解像度: 2.2 Å
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拘束条件 |
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ソフトウェア | *PLUS 名称: PROFT / 分類: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化 | *PLUS Rfactor obs: 0.183 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 | *PLUS タイプ: x_angle_d / Dev ideal: 0.055 |