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- PDB-1lei: The kB DNA sequence from the HLV-LTR functions as an allosteric r... -
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Basic information
Entry | Database: PDB / ID: 1lei | ||||||
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Title | The kB DNA sequence from the HLV-LTR functions as an allosteric regulator of HIV transcription | ||||||
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![]() | TRANSCRIPTION/DNA / transcription factor / NF-kB-DNA complex / TRANSCRIPTION-DNA COMPLEX | ||||||
Function / homology | ![]() cellular response to diterpene / cellular response to glucoside / SUMOylation of immune response proteins / Regulated proteolysis of p75NTR / Interleukin-1 processing / DEx/H-box helicases activate type I IFN and inflammatory cytokines production / RIP-mediated NFkB activation via ZBP1 / MAP3K8 (TPL2)-dependent MAPK1/3 activation / TRAF6 mediated NF-kB activation / positive regulation of hyaluronan biosynthetic process ...cellular response to diterpene / cellular response to glucoside / SUMOylation of immune response proteins / Regulated proteolysis of p75NTR / Interleukin-1 processing / DEx/H-box helicases activate type I IFN and inflammatory cytokines production / RIP-mediated NFkB activation via ZBP1 / MAP3K8 (TPL2)-dependent MAPK1/3 activation / TRAF6 mediated NF-kB activation / positive regulation of hyaluronan biosynthetic process / NF-kB is activated and signals survival / PKMTs methylate histone lysines / Activation of NF-kappaB in B cells / TAK1-dependent IKK and NF-kappa-B activation / cellular response to carbohydrate stimulus / antibacterial innate immune response / mammary gland involution / positive regulation of chondrocyte differentiation / FCERI mediated NF-kB activation / CLEC7A (Dectin-1) signaling / Interleukin-1 signaling / acetaldehyde metabolic process / prolactin signaling pathway / cellular response to interleukin-17 / Downstream TCR signaling / NF-kappaB p50/p65 complex / positive regulation of Schwann cell differentiation / cellular response to peptide / CD209 (DC-SIGN) signaling / cellular response to peptidoglycan / negative regulation of interleukin-12 production / ankyrin repeat binding / negative regulation of protein sumoylation / defense response to tumor cell / postsynapse to nucleus signaling pathway / nucleotide-binding oligomerization domain containing 2 signaling pathway / cellular response to interleukin-6 / cellular response to dsRNA / negative regulation of non-canonical NF-kappaB signal transduction / actinin binding / cellular response to angiotensin / response to UV-B / NF-kappaB complex / positive regulation of leukocyte adhesion to vascular endothelial cell / interleukin-1-mediated signaling pathway / vascular endothelial growth factor signaling pathway / negative regulation of cytokine production / positive regulation of miRNA metabolic process / non-canonical NF-kappaB signal transduction / toll-like receptor 4 signaling pathway / cellular response to hepatocyte growth factor stimulus / positive regulation of amyloid-beta formation / response to cobalamin / positive regulation of T cell receptor signaling pathway / phosphate ion binding / cellular response to cytokine stimulus / cellular response to lipoteichoic acid / response to muramyl dipeptide / general transcription initiation factor binding / cellular response to organic cyclic compound / neuropeptide signaling pathway / NF-kappaB binding / hair follicle development / cellular response to interleukin-1 / positive regulation of vascular endothelial growth factor production / positive regulation of transcription initiation by RNA polymerase II / response to amino acid / RNA polymerase II core promoter sequence-specific DNA binding / lymph node development / canonical NF-kappaB signal transduction / JNK cascade / negative regulation of insulin receptor signaling pathway / tumor necrosis factor-mediated signaling pathway / response to cAMP / cellular response to brain-derived neurotrophic factor stimulus / heat shock protein binding / response to muscle stretch / positive regulation of interleukin-12 production / Neutrophil degranulation / negative regulation of angiogenesis / negative regulation of miRNA transcription / liver development / positive regulation of interleukin-1 beta production / response to cytokine / positive regulation of interleukin-8 production / response to ischemia / response to progesterone / negative regulation of extrinsic apoptotic signaling pathway / RNA polymerase II transcription regulatory region sequence-specific DNA binding / animal organ morphogenesis / B cell receptor signaling pathway / response to bacterium / transcription coregulator activity / peptide binding / response to insulin / protein catabolic process / negative regulation of protein catabolic process / response to organic cyclic compound / chromatin DNA binding / cellular response to virus Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Chen-Park, F. / Huang, D.B. / Ghosh, G. | ||||||
![]() | ![]() Title: The kB DNA sequence from the HIV Long Terminal Repeat functions as an allosteric regulator of HIV transcription Authors: Chen-Park, F.E. / Huang, D.B. / Noro, B. / Thanos, D. / Ghosh, G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 149.8 KB | Display | ![]() |
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PDB format | ![]() | 112.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1vkxS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: DNA chain | Mass: 5269.450 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: kB DNA |
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#2: DNA chain | Mass: 5591.812 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: kB DNA |
#3: Protein | Mass: 31115.236 Da / Num. of mol.: 1 / Fragment: p65 RHR Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#4: Protein | Mass: 35056.066 Da / Num. of mol.: 1 / Fragment: p50 RHR Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.86 Å3/Da / Density % sol: 68.13 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 291 K / Method: small tubes / pH: 6.5 Details: PEG 3350, CaCl2, sodium spermine, pH 6.5, SMALL TUBES, temperature 291K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS Temperature: 18 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 110 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Aug 8, 1999 |
Radiation | Monochromator: MIRRORS / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→30 Å / Num. obs: 33470 / % possible obs: 98 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 1 / Redundancy: 7.6 % / Rmerge(I) obs: 0.064 / Net I/σ(I): 13.8 |
Reflection shell | Resolution: 2.7→2.8 Å / Rmerge(I) obs: 0.31 / Mean I/σ(I) obs: 2.5 / % possible all: 89 |
Reflection | *PLUS % possible obs: 98 % / Num. measured all: 254045 |
Reflection shell | *PLUS % possible obs: 89 % / Rmerge(I) obs: 0.315 |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1VKX Resolution: 2.7→20 Å / Isotropic thermal model: Isotropic / σ(F): 2 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.7→20 Å
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Refine LS restraints |
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Refinement | *PLUS % reflection Rfree: 5 % / Rfactor Rwork: 0.25 | ||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||
Refine LS restraints | *PLUS Type: c_bond_d / Dev ideal: 0.0072 |