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- PDB-1kys: Crystal Structure of a Zn-bound Green Fluorescent Protein Biosensor -
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Open data
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Basic information
Entry | Database: PDB / ID: 1kys | ||||||
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Title | Crystal Structure of a Zn-bound Green Fluorescent Protein Biosensor | ||||||
![]() | Green Fluorescent Protein | ||||||
![]() | LUMINESCENT PROTEIN / beta barrel / chromophore / Zn binding design | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Barondeau, D.P. / Kassmann, C.J. / Tainer, J.A. / Getzoff, E.D. | ||||||
![]() | ![]() Title: Structural chemistry of a green fluorescent protein Zn biosensor. Authors: Barondeau, D.P. / Kassmann, C.J. / Tainer, J.A. / Getzoff, E.D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 117 KB | Display | ![]() |
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PDB format | ![]() | 88.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1kypC ![]() 1kyrC ![]() 1emaS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 26712.008 Da / Num. of mol.: 1 / Mutation: F64L/S65T/Y66H/F99S/Y145F/H148G/M153T/V163A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||||
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#2: Chemical | ChemComp-ZN / #3: Water | ChemComp-HOH / | Sequence details | RESIDUES 65SER AND 66TYR ARE MUTATED TO 65THR AND 66HIS. 65THR, 66HIS AND 67GLY ARE MODIFIED TO ...RESIDUES 65SER AND 66TYR ARE MUTATED TO 65THR AND 66HIS. 65THR, 66HIS AND 67GLY ARE MODIFIED TO MAKE THE CHROMOPHOR | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.86 Å3/Da / Density % sol: 39.97 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 8 Details: PEG 4000, magnesium chloride, hepes, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Details: used microseeding | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Dec 15, 2000 |
Radiation | Monochromator: Curved Crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97 Å / Relative weight: 1 |
Reflection | Resolution: 1.44→1.5 Å / Num. all: 115486 / Num. obs: 110767 / % possible obs: 95.5 % / Observed criterion σ(I): 1 / Redundancy: 3 % / Biso Wilson estimate: 16.3 Å2 / Rsym value: 0.054 / Net I/σ(I): 23.4 |
Reflection shell | Resolution: 1.44→1.49 Å / Mean I/σ(I) obs: 2.4 / Rsym value: 0.316 / % possible all: 79.2 |
Reflection | *PLUS Lowest resolution: 20 Å / Num. obs: 38864 / Num. measured all: 115486 / Rmerge(I) obs: 0.054 |
Reflection shell | *PLUS % possible obs: 79.2 % / Rmerge(I) obs: 0.316 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1ema Resolution: 1.44→20 Å / Num. parameters: 18410 / Num. restraintsaints: 22408 / Cross valid method: FREE R / σ(F): 0 / Stereochemistry target values: ENGH AND HUBER
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Solvent computation | Solvent model: MOEWS & KRETSINGER, J.MOL.BIOL.91(1973)201-22 | |||||||||||||||||||||||||||||||||
Refine analyze | Num. disordered residues: 3 / Occupancy sum hydrogen: 1731 / Occupancy sum non hydrogen: 2034 | |||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.44→20 Å
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Refine LS restraints |
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Software | *PLUS Name: SHELXL / Version: 97 / Classification: refinement | |||||||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 20 Å / Rfactor obs: 0.157 / Rfactor Rfree: 0.229 / Rfactor Rwork: 0.157 | |||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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